1
Kimihiro Shimizu, Tsuguji Nakahara, Taketoshi Kinoshita, Jun Takatsuka, Michiko Igarashi: Plasminogen activator derivatives. Nippon Chemiphar Company, Oblon Fisher Spivak McClelland & Maier, February 3, 1987: US04640835 (346 worldwide citation)

Derivatives of a nonimmunogenic plasminogen activator which comprises at least one polyalkylene glycol group chemically bonded with at least one coupling agent to amino acid side chains of said plasminogen activator, wherein said polyalkylene glycol has a molecular weight of about 200-20,000 and is ...


2
Kimihiro Shimizu, Tsuguji Nakahara, Taketoshi Kinoshita: Plasminogen activator derivatives. January 22, 1985: US04495285 (91 worldwide citation)

A derivative of a human-originated non-immunogenic plasminogen activator is disclosed which comprises at least one polyalkylene glycol attached with at least one coupling agent to amino acid side chains of said plasminogen activator, said polyalkylene glycol having a molecular weight of 200-20,000 a ...


3
Nils U Bang, Sheila P Little, Brigitte E Schoner, Barbara J Weigel: DNA encoding novel tissue plasminogen activator derivatives having kringles 1 and 2 deleted, vectors and host cells. Eli Lilly and Company, Thomas G Plant, Leroy Whitaker, September 14, 1993: US05244806 (4 worldwide citation)

The invention discloses modified forms of the enzyme tissue plasminogen activator (t-PA) in which recombinant DNA techniques are utilized to modify the structure of the t-PA cDNA to express a modified t-PA which retains the ability to activate plasminogen yet binds less efficiently to plasmin inhibi ...


4
David V Goeddel, William J Kohr, Diane Pennica, Gordon A Vehar: Methods of preparing tissue plasminogen activator derivatives. Genentech, Walter H Dreger, March 17, 1998: US05728565 (1 worldwide citation)

Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-terminal first 68 amino acids is specifically ...


5
David V Goeddel, William J Kohr, Diane Pennica, Gordon A Vehar: Tissue plasminogen activator derivatives. Genentech, Walter H Dreger, March 17, 1998: US05728566 (1 worldwide citation)

Tissue plasminogen activator (t-PA) derivatives are produced in useful quantifies using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-terminal first 68 amino acids is specifically ...


6
Mackellar Warren Cameron, Robey Carolyn Sue: An improved method for folding tissue plasminogen activators and derivatives thereof.. Lilly Co Eli, January 20, 1993: EP0523296-A1

The present invention discloses and claims the addition of lysine to protein folding procedures to improve the yield of substrate specific, enzymatically active tissue plasminogen activator or tissue plasminogen activator derivatives comprising kringle-2.


7
Shimizu Kimihiro, Nakahara Tsuguji, Kinoshita Taketoshi: Derives dun activateur du plasminogene, Plasminogen activator derivatives. Nippon Chemiphar, GOWLING LAFLEUR HENDERSON, April 29, 1986: CA1203764

ABSTRACT OF THE DISCLOSURE A derivative of a human-originated non-immunogenicplasminogen activator is disclosed which comprises atleast one polyalkylene glycol attached with at least onecoupling agent to amino acid side chains of said plasmino-gen activator, said polyalkylene glycol having a molecul ...


8
Shimizu Kimihiro, Nakahara Tsuguji, Kinoshita Taketoshi: Plasminogen activator derivatives. Nippon Chemiphar Co, June 15, 1983: GB2110219-A

NOUVEAU DERIVE D'UN ACTIVATEUR DE PLASMINOGENE NON IMMUNOGENE D'ORIGINE HUMAINE. CE DERIVE COMPREND AU MOINS UN POLYALCOYLENE GLYCOL FIXE AVEC AU MOINS UN AGENT DE COUPLAGE AUX CHAINES LATERALES AMINO-ACIDE DE CET ACTIVATEUR DE PLASMINOGENE, LE POLYALCOYLENE GLYCOL POSSEDANT UN POIDS MOLECULAIRE SE ...


9
Kohnert Ulrich, Stern Anne, Martin Ulrich, Fischer Stephan: (De) Nicht-glykosylierte plasminogenaktivator-derivate und ihre verwendung bei erhöhtem blutungsrisiko, (En) Non-glycosylated plasminogen activator derivatives and their use in conditions involving a high risk of bleeding. Boehringer Mannheim, Kohnert Ulrich, Stern Anne, Martin Ulrich, Fischer Stephan, January 18, 1996: WO/1996/001312

(EN) The invention concerns a non-glycosylated tissue plasminogen activator derivative which contains the essential parts of at least one Kringle domain and a B-chain with serine protease activity of the tissue plasminogen activator and which is characterized in that each of the amino-acids 296 to 2 ...


10
Miyashita Sadakazu, Kasai Shunji, Hashimoto Akira: Sugar chain-deficient tissue plasminogen activator derivatives. Eisai, Miyashita Sadakazu, Kasai Shunji, Hashimoto Akira, FURUYA Kaoru, June 5, 1997: WO/1997/020042

Human tissue plasminogen activator (t-PA) derivatives wherein the chain structure is altered so as to elevate the activity. A t-PA derivative constructed by substituting Cys 84 by Ser is treated with combinations of various sugar-cleaving enzymes to thereby give derivatives having different sugar ch ...