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Arnold Caplan, Glenn T Syftestad: Bone protein purification process. Watts Hoffmann Fisher & Heinke Co, August 26, 1986: US04608199 (69 worldwide citation)

A process of extracting and purifying a bone protein capable of stimulating chondrogenic expression in undifferentiated cells in culture. The purification process is monitored at various stages by bioassaying the bone protein for chondrogenic activity in embryonic limb bud mesenchymal cell cultures.


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Caplan Arnold I, Syftestad Glenn T: Bone protein purification process. Caplan Arnold I, Syftestad Glenn T, HEINKE Lowell L, September 26, 1985: WO/1985/004173

A process of extracting and purifying a bone protein capable of stimulating chondrogenic expression in undifferentiated cells in culture. The purification process is monitored at various stages by bioassaying the bone protein for chondrogenic activity in embryonic limb bud mesenchymal cell cultures.