61
Robert A LaRossa: Yeast strain for production of four carbon alcohols. Butamax Advanced Biofuels, January 27, 2015: US08940511 (17 worldwide citation)

Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protei ...


62
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, November 25, 2014: US08895307 (17 worldwide citation)

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.


63
Michael J Haas: Fuels as solvents for the conduct of enzymatic reactions. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John Fado, Janelle S Graeter, December 16, 1997: US05697986 (17 worldwide citation)

The present invention describes a method of producing biofuels by carrying out the enzymatic transesterification of fatty acid-containing materials directly in automotive fuels.


64
Aniket Kale: Methods of dewatering algae for extraction of algal products. Heliae Development, Tom Gallegos Esq, Justin Kniep Esq, Wilmer Cutler Pickering Hale and Dorr, November 27, 2012: US08318019 (16 worldwide citation)

A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid remov ...


65
Larry Cameron Anthony, Lori Ann Maggio Hall: Enhanced pyruvate to acetolactate conversion in yeast. Butamax Advanced Biofuels, March 3, 2015: US08969065 (16 worldwide citation)

A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activit ...


66
Zhihao Hu, Fernando Valle: Enhanced production of fatty acid derivatives. LS9, Linda R Judge, LS9, February 7, 2012: US08110670 (16 worldwide citation)

Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.


67
Susan Leschine, Thomas A Warnick: Systems and methods for producing biofuels and related materials. University of Massachusetts, Fish & Richardson P C, March 23, 2010: US07682811 (16 worldwide citation)

Clostridium phytofermentans cells (American Type Culture Collection 700394T) and all other strains of the species can ferment materials such as biomass into useful products and coproducts, such as ethanol, hydrogen and organic acids. Compositions that include Clostridium phytofermentans are also dis ...


68
Marc A Portnoff, David A Purta, Margaret A Nasta, Jingfeng Zhang, Faiz Pourarian: Methods for producing biodiesel. Carnegie Mellon University, Michael Best & Friedrich, October 18, 2011: US08039652 (15 worldwide citation)

Transesterification, esterification, and esterification-transesterification (both one-step and two-step) for producing biofuels. The process may be enhanced by one or more of the following: 1) applying microwave or RF energy; 2) passing reactants over a heterogeneous catalyst at sufficiently high ve ...


69
Michael Cheiky, Rajashekharam Malyala: System for making renewable fuels. Cool Planet Biofuels, Sheppard Mullin Richter & Hampton, David E Heisey, March 20, 2012: US08137628 (15 worldwide citation)

Multiple catalytic processing stations couple with a system which produces volatile gas streams from biomass decomposition at discrete increasing temperatures. These catalytic processing stations can be programmed to maximize conversion of biomass to useful renewable fuel components based on input f ...


70
KALE Aniket: PROCÉDÉS DE PRODUCTION DE BIOCARBURANTS, DE CHLOROPHYLLES ET DE CAROTÉNOÏDES, METHODS OF PRODUCING BIOFUELS, CHLOROPHYLLS AND CAROTENOIDS. HELIAE DEVELOPMENT, KALE Aniket, SUPERKO Colleen, October 11, 2012: WO/2012/138438 (15 worldwide citation)

A method for producing biofuels along with valuable food and neutraceutical products is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and s ...



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