61
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, November 25, 2014: US08895307 (17 worldwide citation)

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.


62
Michael J Haas: Fuels as solvents for the conduct of enzymatic reactions. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John Fado, Janelle S Graeter, December 16, 1997: US05697986 (17 worldwide citation)

The present invention describes a method of producing biofuels by carrying out the enzymatic transesterification of fatty acid-containing materials directly in automotive fuels.


63
Aniket Kale: Methods of dewatering algae for extraction of algal products. Heliae Development, Tom Gallegos Esq, Justin Kniep Esq, Wilmer Cutler Pickering Hale and Dorr, November 27, 2012: US08318019 (16 worldwide citation)

A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid remov ...


64
Larry Cameron Anthony, Lori Ann Maggio Hall: Enhanced pyruvate to acetolactate conversion in yeast. Butamax Advanced Biofuels, March 3, 2015: US08969065 (16 worldwide citation)

A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activit ...


65
Gail K Donaldson, Andrew C Eliot, Vasantha Nagarajan, Charles E Nakamura, Jean Francois Tomb: Fermentive production of four carbon alcohols. Butamax Advanced Biofuels, March 17, 2015: US08980612 (16 worldwide citation)

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be ...


66
Susan Leschine, Thomas A Warnick: Systems and methods for producing biofuels and related materials. University of Massachusetts, Fish & Richardson P C, March 23, 2010: US07682811 (16 worldwide citation)

Clostridium phytofermentans cells (American Type Culture Collection 700394T) and all other strains of the species can ferment materials such as biomass into useful products and coproducts, such as ethanol, hydrogen and organic acids. Compositions that include Clostridium phytofermentans are also dis ...


67
Marc A Portnoff, David A Purta, Margaret A Nasta, Jingfeng Zhang, Faiz Pourarian: Methods for producing biodiesel. Carnegie Mellon University, Michael Best & Friedrich, October 18, 2011: US08039652 (15 worldwide citation)

Transesterification, esterification, and esterification-transesterification (both one-step and two-step) for producing biofuels. The process may be enhanced by one or more of the following: 1) applying microwave or RF energy; 2) passing reactants over a heterogeneous catalyst at sufficiently high ve ...


68
KALE Aniket: PROCÉDÉS DE PRODUCTION DE BIOCARBURANTS, DE CHLOROPHYLLES ET DE CAROTÉNOÏDES, METHODS OF PRODUCING BIOFUELS, CHLOROPHYLLS AND CAROTENOIDS. HELIAE DEVELOPMENT, KALE Aniket, SUPERKO Colleen, October 11, 2012: WO/2012/138438 (15 worldwide citation)

A method for producing biofuels along with valuable food and neutraceutical products is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids along with carotenoids and chlorophylls from the algal biomass, and s ...


69
Atalla Rajai H: Highly disordered cellulose. Cellulose Sciences International, Atalla Rajai H, WELCH Teresa J, October 8, 2009: WO/2009/124240 (15 worldwide citation)

Disclosed are methods and systems for treating cellulose to make it more accessible for enzymatic or chemical modification. The invention includes treating cellulose with an alkali in an alcohol/water co-solvent system. The treatment decrystallizes or deaggregates the cellulosic material. The method ...


70
Michael Cheiky, Rajashekharam Malyala: System for making renewable fuels. Cool Planet Biofuels, Sheppard Mullin Richter & Hampton, David E Heisey, March 20, 2012: US08137628 (14 worldwide citation)

Multiple catalytic processing stations couple with a system which produces volatile gas streams from biomass decomposition at discrete increasing temperatures. These catalytic processing stations can be programmed to maximize conversion of biomass to useful renewable fuel components based on input f ...



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