61
Gail K Donaldson, Andrew C Eliot, Vasantha Nagarajan, Charles E Nakamura, Jean Francois Tomb: Recombinant host cell comprising a diol dehydratase. Butamax Advanced Biofuels, February 24, 2015: US08962298 (13 worldwide citation)

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be ...


62
Dennis Flint, Steven Cary Rothman, Wonchul Suh, Jean Francois Tomb, Rick W Ye: Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases. Butamax Advanced Biofuels, February 10, 2015: US08951937 (13 worldwide citation)

A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvaler ...


63
Michael Dauner, Lori Ann Maggio Hall, Jean Francois Tomb: Recombinant host cells comprising phosphoketolases. Butamax Advanced Biofuels, Andrew P Baraniak, October 28, 2014: US08871488 (13 worldwide citation)

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polyp ...


64
Keith Alsaker, Daniel Grenfell Lee, Michael Hudson, Adam Lawrence, Jessica McGrath, David Young: Engineering resistance to aliphatic alcohols. Butamax Advanced Biofuels, December 9, 2014: US08906666 (13 worldwide citation)

The present disclosure provides improved systems for the biological production of aliphatic alcohol compounds. In particular, the present disclosure provides biological systems that show improved resistance to aliphatic alcohol toxicity; in some embodiments, such improved resistance allows for incre ...


65
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, December 16, 2014: US08911981 (13 worldwide citation)

Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.


66
Robert A LaRossa: Yeast strain for production of four carbon alcohols. Butamax Advanced Biofuels, January 27, 2015: US08940511 (13 worldwide citation)

Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protei ...


67
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, November 25, 2014: US08895307 (13 worldwide citation)

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.


68
Yoshikuni Yasuo, Kashiyama Yuki: Biofuel production. Bio Architecture Lab, Yoshikuni Yasuo, Kashiyama Yuki, CHRISTIANSEN William T, April 9, 2009: WO/2009/046375 (13 worldwide citation)

Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such ...


69
Michael Cheiky, Rajashekharam Malyala: System for making renewable fuels. Cool Planet Biofuels, Sheppard Mullin Richter & Hampton, David E Heisey, March 20, 2012: US08137628 (12 worldwide citation)

Multiple catalytic processing stations couple with a system which produces volatile gas streams from biomass decomposition at discrete increasing temperatures. These catalytic processing stations can be programmed to maximize conversion of biomass to useful renewable fuel components based on input f ...


70
Larry Cameron Anthony, Lori Ann Maggio Hall: Enhanced pyruvate to acetolactate conversion in yeast. Butamax Advanced Biofuels, March 3, 2015: US08969065 (12 worldwide citation)

A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activit ...



Click the thumbnails below to visualize the patent trend.