51
Gail K Donaldson, Andrew C Eliot, Vasantha Nagarajan, Charles E Nakamura, Jean Francois Tomb: Recombinant host cell comprising a diol dehydratase. Butamax Advanced Biofuels, February 24, 2015: US08962298 (19 worldwide citation)

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be ...


52
Dennis Flint, Steven Cary Rothman, Wonchul Suh, Jean Francois Tomb, Rick W Ye: Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases. Butamax Advanced Biofuels, February 10, 2015: US08951937 (19 worldwide citation)

A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvaler ...


53
Keith Alsaker, Daniel Grenfell Lee, Michael Hudson, Adam Lawrence, Jessica McGrath, David Young: Engineering resistance to aliphatic alcohols. Butamax Advanced Biofuels, December 9, 2014: US08906666 (19 worldwide citation)

The present disclosure provides improved systems for the biological production of aliphatic alcohol compounds. In particular, the present disclosure provides biological systems that show improved resistance to aliphatic alcohol toxicity; in some embodiments, such improved resistance allows for incre ...


54
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, December 16, 2014: US08911981 (19 worldwide citation)

Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.


55
Robert A LaRossa: Yeast strain for production of four carbon alcohols. Butamax Advanced Biofuels, January 27, 2015: US08940511 (19 worldwide citation)

Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protei ...


56
Yougen Li, Der Ing Liao, Mark J Nelson, Daniel P Okeefe: Ketol-acid reductoisomerase using NADH. Butamax Advanced Biofuels, November 25, 2014: US08895307 (19 worldwide citation)

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.


57
Michael J Haas: Fuels as solvents for the conduct of enzymatic reactions. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, John Fado, Janelle S Graeter, December 16, 1997: US05697986 (19 worldwide citation)

The present invention describes a method of producing biofuels by carrying out the enzymatic transesterification of fatty acid-containing materials directly in automotive fuels.


58
Larry Cameron Anthony, Lori Ann Maggio Hall: Enhanced pyruvate to acetolactate conversion in yeast. Butamax Advanced Biofuels, March 3, 2015: US08969065 (18 worldwide citation)

A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activit ...


59
Liao James C, Atsumi Shota, Smith Kevin M, Shen Roa Pu Claire, Cann Anthony F, Connor Michael R: Biofuel production by recombinant microorganisms. The Regents Of The University Of California, Liao James C, Atsumi Shota, Smith Kevin M, Shen Roa Pu Claire, Cann Anthony F, Connor Michael R, BAKER Joseph R Jr, August 14, 2008: WO/2008/098227 (18 worldwide citation)

Provided herein are metabolically-modif ied microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-l-butanol, 3- methyl-1-butanol and 2-phenylethanol from a suitable substrate.


60
Aniket Kale, Luca Costantino Zullo, Sandip Shinde: Methods of and systems for producing biofuels from algal oil. Heliae Development, Tom Gallegos Esq, Justin Kniep Esq, Wilmer Cutler Pickering Hale and Dorr, November 20, 2012: US08313648 (17 worldwide citation)

A method for producing biofuels is provided that includes dewatering intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fr ...



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