41
Rainer Potthast, Chi Ping Chung, Indresh Mathur: Purification of glycerin obtained as a bioproduct from the transesterification of triglycerides in the synthesis of biofuel. Johann Haltermann, Butzel Long, May 18, 2010: US07718833 (23 worldwide citation)

Methods for purifying glycerin contaminated with one or more lower boiling alcohols such as methanol, ethanol, straight, branched or cyclic C3-C6 alcohols, and the like. The methods are particularly useful for purifying crude glycerin phases recovered from the synthesis of biofuels. The present inve ...


42
Michael G Bramucci, Andrew C Eliot, Lori Ann Maggio Hall, Charles E Nakamura: Butanol dehydrogenase enzyme from the bacterium. Butamax Advanced Biofuels, Christine M Lhulier, April 8, 2014: US08691540 (22 worldwide citation)

From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butano ...


43
Michael Dauner, Lori Ann Maggio Hall, Jean Francois Tomb: Recombinant host cells comprising phosphoketolases. Butamax Advanced Biofuels, Andrew P Baraniak, October 28, 2014: US08871488 (22 worldwide citation)

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polyp ...


44
Michael G Bramucci, Robert A Larossa, Dana R Smulski: Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway. Butamax Advanced Biofuels, Christine M Lhulier, June 4, 2013: US08455225 (22 worldwide citation)

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the high osmolarity/glycerol response pathway. Yeast with increased expression of PBS2p, a mitogen activated protein kinase kinase of the MAPK module of the high osmolarity/glycerol response pathway may be used ...


45
LIPPMEIER JAMES CASEY: Recombinant thraustochytrids that grow on xylose, and compositions, methods of making, and uses thereof. MARTEK BIOSCIENCES CORPORATION, Kim Judith U, July 28, 2011: WO/2011/090730 (22 worldwide citation)

The present invention is directed to recombinant thraustochytrids that grow on xylose and cell cultures comprising the recombinant thraustochytrids as well as methods of producing cell cultures, biomasses, microbial oils, compositions, and biofuels using the recombinant thraustochytrids.


46
Gail K Donaldson, Andrew C Eliot, Dennis Flint, Lori Ann Maggio Hall, Vasantha Nagarajan: Fermentive production of four carbon alcohols. Butamax Advanced Biofuels, February 10, 2015: US08951774 (21 worldwide citation)

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.


47
Larry Cameron Anthony, Lori Ann Maggio Hall: Enhanced pyruvate to acetolactate conversion in yeast. Butamax Advanced Biofuels, February 17, 2015: US08956850 (20 worldwide citation)

A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activit ...


48
Gail K Donaldson, Andrew C Eliot, Vasantha Nagarajan, Charles E Nakamura, Jean Francois Tomb: Recombinant host cell comprising a diol dehydratase. Butamax Advanced Biofuels, February 24, 2015: US08962298 (20 worldwide citation)

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be ...


49
Gail K Donaldson, Andrew C Eliot, Vasantha Nagarajan, Charles E Nakamura, Jean Francois Tomb: Fermentive production of four carbon alcohols. Butamax Advanced Biofuels, March 17, 2015: US08980612 (20 worldwide citation)

Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be ...


50
Dennis Flint, Steven Cary Rothman, Wonchul Suh, Jean Francois Tomb, Rick W Ye: Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases. Butamax Advanced Biofuels, February 10, 2015: US08951937 (20 worldwide citation)

A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvaler ...



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