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David Mosley
Peter A Carr, Brian Y Chow, Joseph M Jacobson, David W Mosley, Christopher Emig: Methods for High Fidelity Production of Long Nucleic Acid Molecules with Error Control. Massachusetts Institute of Technology, January 3, 2013: US20130005612-A1

A method for synthesizing a nucleic acid having a desired sequence and length comprises providing a solid support having an immobilized nucleic acid, performing a nucleic acid addition reaction to elongate the immobilized nucleic acid by adding a nucleotide or an oligonucleotide attached to a protec ...


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David Mosley
Peter A Carr, Brian Y Chow, Joseph M Jacobson, David W Mosley, Christopher Emig: Methods for High Fidelity Production of Long Nucleic Acid Molecules. Massachusetts Institute of Technology, October 18, 2012: US20120264653-A1

In a method for synthesizing a pool of nucleic acid molecules, a first nucleic acid has a first 5′ region and a first 3′ region and a second nucleic acid has a second 5′ region and a second 3′ region. The second 3′ region and the first 5′ region have identical nucleic acid sequences. The first 3′ re ...


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Stemmer Willem P C, Crameri Andreas: Methods for generating polynucleotides having desired characteristics by iterative selective and recombination. Maxygen, April 28, 1999: EP0911396-A2 (95 worldwide citation)

The present invention relates to a method for evolving a polynucleotide encoding a plurality of genes, e.g. multiple genes forming a multicomponent pathway. The method involves shuffling of polynucleotides by conducting a polynucleotide amplification process on overlapping segments of a population o ...


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ROTHBERG JONATHAN M: Methods and apparatus for measuring analytes. ION TORRENT SYSTEMS INCORPORATED, Trevisan Maria A, December 2, 2010: WO/2010/138182 (89 worldwide citation)

Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by- synthesis reactions. Some methods provided herein relate to improving signal (and also signal-to-noise ratio) from released hydrogen ions du ...


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Stemmer Willem P C, Crameri Andreas: Dna mutagenesis by random fragmentation and reassembly. Affymax Tech, January 8, 1997: EP0752008-A1 (69 worldwide citation)

The invention is directed to methods of shuffling polynucleotide variants. The methods entail conducting a multi-cyclic polynucleotide extension process on partially annealed polynucleotide strands having sequences from the plurality of chosen polynucleotide variants, the polynucleotide strands havi ...


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Pirrung Michael C, Read J Leighton, Fodor Stephen P A, Stryer Lubert: Very large scale immobilized peptide synthesis.. Affymax Tech, October 12, 1994: EP0619321-A1 (66 worldwide citation)

A method of preparing preselected chemical sequences at known locations on a single substrate surface, comprising (a) exposing a selected and reactive region of the substrate surface, which region may or may not carry a monomer or monomer sequence, to an activator to enable the region to react with ...


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Seed Brian Dr Department Of Mo, Allen Janet, Aruffo Alejandro, Camerini David, Lauffer Leander Dr, Oquendo Carmen Patricia, Simmons David, Stamenkovic Ivan, Stengelin Siegfried Dr: Rapid immunoselection cloning method.. Gen Hospital, August 30, 1989: EP0330191-A2 (59 worldwide citation)

A simple and highly efficient method for cloning cDNAs from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the i ...


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Ladner Robert Charles, Guterman Sonia K: Generation and selection of recombinant varied binding proteins.. Protein Eng, July 17, 1991: EP0436597-A1 (57 worldwide citation)

In order to obtain a novel binding protein against a chosen target, DNA molecules, each encoding a protein comprising one of a family of similar potential binding domains and a structural signal calling for the display of the protein on the outer surface of a chosen bacterial cell, bacterial spore o ...


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CHURCH George M, VIGNEAULT Francois: ÉTABLISSEMENT À HAUT DÉBIT DUN CODE-BARRES DE CELLULES SIMPLES, HIGH-THROUGHPUT SINGLE CELL BARCODING. PRESIDENT AND FELLOWS OF HARVARD COLLEGE, CHURCH George M, VIGNEAULT Francois, IWANICKI John P, April 12, 2012: WO/2012/048341 (53 worldwide citation)

Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.


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