1
Kleid Dennis G, Yansura Daniel G, Heyneker Herbert L, Miozzari Giuseppe F: A method of creating an expression plasmid.. Genentech, September 30, 1981: EP0036776-A2 (254 worldwide citation)

Novel plasmidic expression vehicles and methods of using them in the production of useful polypeptides by recombinant bacteria are described. The plasmids employ a tryptophan promoter-operator system from which the attenuator region ordinarily present has been deleted. Bacteria containing the plasmi ...


2
Ward David C, Waldrop Alexander A Iii, Langer Pennina R: Modified nucleotides and methods of preparing and using same.. Univ Yale, November 3, 1982: EP0063879-A2 (213 worldwide citation)

Compounds having the structure: wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C-position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N-position of the purine or 7-deazapurine and when B is pyrimidine, it is ...


3
Nakagawa Satochi, Mizoguchi Hiroshi, Ando Seiko, Hayashi Mikiro, Ochiai Keiko, Yokoi Haruhiko, Tateishi Naoko, Senoh Akihiro, Ikeda Masato, Ozaki Akio: Novel polynucleotides. Kyowa Hakko Kogyo, June 20, 2001: EP1108790-A2 (180 worldwide citation)

Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences o ...


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Erlich Henry Anthony, Horn Glenn, Saiki Randall Keichi, Stoffel Susanne, Mullis Kary Banks, Lawyer Frances Cook, Gelfand David Harrow: Purified thermostable enzyme and process for amplifying, detecting, and/or cloning nucleic acid sequences using said enzyme.. Cetus, March 2, 1988: EP0258017-A2 (150 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


6
Emil C Gotschlich: Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them. The Rockefeller University, Klauber & Jackson, August 13, 1996: US05545553 (101 worldwide citation)

The present invention is directed to nucleic acids encoding glycosyltransferases, the proteins encoded thereby, and to methods for synthesizing oligosaccharides using the glycosyltransferases of the invention. In particular, the present application is directed to identification a glycosyltransferase ...


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Diderichsen Borge Krag, Outtrup Helle, Schulein Martin, Norman Barrie Edmund: Chimeric enzymes.. Novo Industri, January 13, 1988: EP0252666-A2 (94 worldwide citation)

Chimeric alpha-amylases are disclosed of the formula Q-R-L in which Q is a N-terminal polypeptide residue of from 55 to 60 amino acid residues which is at least 75 percent homologous to the 55 N-terminal amino acid residues in the Bacillus amyloliquefaciens alpha-amylase as described in Takkinen et ...


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Gerlach Wayne Lyle, Haseloff James Phillip, Jennings Philip Anthony, Cameron Fiona Helen: Ribozymes.. Commw Scient Ind Res, June 21, 1989: EP0321201-A2 (92 worldwide citation)

Synthetic RNA molecules having highly specific endoribonuclease activity (hereinafter referred to as "ribozymes") are described. The ribozymes comprise a hybridizing region which is complementary in nucleotide sequence to at least part of a target RNA, and a catalytic region which is adapted to clea ...


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Mossa Albert Carmelo, Goven Lisa May, Atwood John Girdner, Williams Fenton, Woudenberg Timothy M, Margulies Marcel, Ragusa Robert P, Leath Richard: Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control.. Perkin Elmer Cetus Instr, June 3, 1992: EP0488769-A2 (90 worldwide citation)

An instrument for performing highly accurate PCR employing a sample block in microtiter tray format. The sample block has local balance and local symmetry. A three zone film heater controlled by a computer and ramp cooling solenoid valves also controlled by the computer for gating coolant flow throu ...


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Beppu Teruhiko, Uozumi Takeshi, Nishimori Katsuhiko, Shimizu Norio, Kawaguchi Yoshiyuki, Hidaka Makoto: Novel expression plasmids and their use in the method for expressing prochymosin coding gene in e. coli.. Beppu Teruhiko, October 17, 1984: EP0121775-A1 (79 worldwide citation)

Improved methods are provided for replication and expression of prochymosin coding gene in E. coli. Novel expression plasmids having prochymosin coding gene operatively attached to the E. coli trp operon have been developed. These plasmids have been inserted into E. coli host cells by transformation ...