1
Irina A Nazarenko, Satish K Bhatnagar, Emily S Winn Deen, Robert J Hohman: Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon. Oncor, Jonathan M Oncor Cohen, February 2, 1999: US05866336 (286 worldwide citation)

The present invention provides labeled nucleic acid amplification oligonucleotides, which can be linear or hairpin primers or blocking oligonucleotides. The oligonucleotides of the invention are labeled with donor and/or acceptor moieties of molecular energy transfer pairs. The moieties can be fluor ...


2
Peter Zaun, Stanley R Bouma, Julian Gordon, John J Kotlarik: Apparatus and method for amplifying and detecting target nucleic acids. Abbott Laboratories, Thomas D Brainard, May 16, 1995: US05415839 (235 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection c ...


3
Dirk M Anderson, Paul E Baker, Michael A Cantrell, Douglas P Cerretti, David J Cosman, Steven D Gimpel, Kenneth H Grabstein, Alf D Larsen, Kate N McKereghan: DNA sequences encoding bovine interleukin-2. Immunex Corporation, Jerald Nagae, Scott G Hallquist, Christopher L Wight, November 21, 1989: US04882282 (4 worldwide citation)

A chemically-synthesized oligonucleotide composing a portion of the nucleotide sequence of the human IL-2 is employed as a probe to isolate the gene coding for human IL-2. The human IL-2 gene is selected from a cDNA library prepared from RNA produced by mitogen-stimulated Jurkat cells. Double-strand ...


4
Kwong Kwok Wong: Methods for producing partially digested restriction DNA fragments and for producing a partially modified PCR product. Battelle Memorial Institute, Paul W Zimmerman, April 4, 2000: US06046039 (1 worldwide citation)

The present invention is an improved method of making a partially modified PCR product from a DNA fragment with a polymerase chain reaction (PCR). In a standard PCR process, the DNA fragment is combined with starting deoxynucleoside triphosphates, a primer, a buffer and a DNA polymerase in a PCR mix ...