1
Nishimura Osamu, Fujino Masahiko: Chemically modified lymphokine and production thereof.. Takeda Chemical Industries, September 11, 1985: EP0154316-A2 (337 worldwide citation)

The invention provides chemically modified lymphokines having R &lparstr& O-CH2CH2 &rparstr& n wherein R is a protective group for the terminal oxygen and n is an optional positive integer, bonded directly to at least one primary amino group of the lymphokine moiety, and a method of producing the sa ...


2
Danute E Nitecki: Preparation of an activated polymer ester for protein conjugation. Cetus Oncology Corporation, Grant D Green, Kenneth M Goldman, Philip L McGarrigle Jr, January 25, 1994: US05281698 (226 worldwide citation)

The present invention is a process for preparing an activated ester of polyethylene glycol or a polyoxyethylated polyol. After the activated ester is prepared, it can be reacted with a protein to form a polymer/protein conjugate. Conjugation with a polymer can reduce the protein's immunogenicity, in ...


3
Robert Karasiewicz, Carlo Nalin, Perry Rosen: Peg-interferon conjugates. Hoffmann La Roche, George M Gould, William H Epstein, Catherine R Roseman, January 17, 1995: US05382657 (204 worldwide citation)

Substituted PEG-interferon conjugates of formulae IA and IB where PEG is linked by means of activated linking reagents of formulae IIA, IIB, or IIB-1 to an amino group in the interferon, and activated linking reagents of formulae IIA, IIB, or IIB-1. The conjugates are not readily susceptible to in v ...


4
David F Mark, Leo S Lin, Shi Da Yu Lu: Human recombinant cysteine depleted interferon-.beta. muteins. Cetus Corporation, Albert P Halluin, Leona L Lauder, Thomas E Ciotti, May 13, 1986: US04588585 (197 worldwide citation)

Muteins of biologically active proteins such as IFN-.beta. and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to eliminate sites for intermolecular crosslinking or incorrect intramolecular disulfide bridge formation. ...


5
Diana K Goroff: In-vitro method for producing antigen-specific human monoclonal antibodies. Akzo, Donna Bobrowicz, July 20, 1993: US05229275 (189 worldwide citation)

A method of amplifying production of IgG, IgA and IgM antigen specific human monoclonal antibodies from transformed, T-cell depleted, human peripheral blood lymphocytes through the use of an adjuvant system consisting of 8-mercaptoguanosine and at least one of the cytokines interleukin-4 and interle ...


6
Leslie D Bell, Keith G McCullagh, Alan G Porter: Covalently linked polypeptide cell modulators. G D Searle and Company, Mary Jo Kanady, Paul D Matukaitis, June 19, 1990: US04935233 (146 worldwide citation)

Described is a new class of polypeptide cell modulators characterized by being composed of two covalently linked cell modulators in a linear polypeptide sequence. Such dual function polypeptides have new and particularly useful activities when the component polypeptide cell modulators are interferon ...


7
Yitzhak Stabinsky: Consensus human leukocyte interferon. Amgen, Marshall O Toole Gerstein Murray & Bicknell, September 22, 1987: US04695623 (123 worldwide citation)

Described are rapid and highly efficient procedures for the total synthesis of linear, double stranded DNA sequences in excess of about 200 base pairs in length, which sequences may comprise entire structural genes. Novel sequences are prepared from two or more DNA subunits provided with terminal re ...


8
Karasiewicz Robert, Nalin Carlo, Rosen Perry: Peg-interferon conjugates.. Hoffmann La Roche, April 27, 1994: EP0593868-A1 (74 worldwide citation)

The present invention relates to physiologically active water soluble polyethylene glycol conjugates with interferon and to new polyethylene glycol compounds which can be used in the preparation of the conjugates.


9
Senoo Masaharu, Onda Haruo, Igarashi Koichi: Dna and use thereof.. Takeda Chemical Industries, October 16, 1985: EP0158198-A1 (65 worldwide citation)

A polypeptide comprising amino acid sequence of human interferon and that of human interleukine-2-may be produced by making new DNA by the ligation of DNA containing the structural gene coding for the peptide of human interferon with DNA containing the structural gene coding for human interleukine-2 ...


10
Leslie D Bell, Keith G McCullagh, Alan G Porter: Covalently linked polypeptide cell modulators such as interferon-lymphotoxin conjugates. G D Searle & Co, Mary Jo Kanady, Paul D Matukaitis, May 19, 1992: US05114711 (59 worldwide citation)

Described is a new class of polypeptide cell modulators characterized by being composed of two covalently linked cell modulators in a linear polypeptide sequence. Such dual function polypeptides have new and particularly useful activities when the component polypeptide cell modulators are interferon ...