1
Beppu Teruhiko, Horinouchi Sueharu, Nishiyama Makoto, Yu Fujio, Hashimoto Yoshihiro: Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids.. Nitto Chemical, Beppu Teruhiko, September 9, 1992: EP0502476-A2 (43 worldwide citation)

The present invention provides a recombinant plasmid comprising combining a hybrid plasmid vector with the isolated DNA sequences of one or more genes encoding nitrile degrading enzymes which are derived from bacteria belonging to the genus Rhodococcus, said hybrid plasmid vector comprising an isola ...


2
Yu Fujio: Novel nitrilase gene. Nitto Chemical, June 25, 1997: EP0780471-A2 (25 worldwide citation)

The invention relates to a DNA coding for a polypeptide having nitrilase activity with an amino acid sequence as shown in SEQ ID NO:1. As compared with conventional processes, the hydrolysis of nitriles by a nitrilase gene cloned by genetic recombination is expected to drastically improve the cataly ...


3
Mizumura Yurie, Yu Fujio: A regulatory factor for expression of nitrilase gene and a gene thereof. Nitto Chemical, July 3, 1996: EP0719862-A2 (4 worldwide citation)

The invention relates to a two component regulatory factor which activates a nitrilase gene promoter, comprising a polypeptide having the amino acid sequence of SEQ ID No: 1 and a polypeptide having the amino acid sequence of SEQ ID No: 2. Nitrilase can be produced by introducing the DNA coding for ...


4
Watanabe Fumiaki, Ujihara Dai, Sakai Miki, Yu Fujio, Nakamura Tetsuji: (Ja) 改良型ニトリルヒドラターゼ, (En) Improved nitrile hydratase. Mitsubishi Rayon, Watanabe Fumiaki, Ujihara Dai, Sakai Miki, Yu Fujio, Nakamura Tetsuji, KOBAYASHI Hiroshi, December 8, 2005: WO/2005/116206 (3 worldwide citation)

(EN) There is provided an improved nitrile hydratase activity having protein that is obtained by modifying the amino acid sequence of nitrile hydratase to thereby have a heat resistance superior to that of the wild-type nitrile hydratase activity. Further, there are provided a gene DNA coding for th ...


5
Akita Takashi, Ito Chiho, Ishimaru Teruta, Miyauchi Haruko, Murase Kei, Takahashi Atsushi, Sumi Toshinori, Maehara Osamu, Ikeda Tadanobu, Oogami Nobuko, Makino Takayuki, Yu Fujio, Watanabe Fumiaki, Uragaki Toshitaka, Fujii Wataru, Morishita Takeharu: Microarrays having biological substance. Mitsubishi Rayon Co, November 28, 2001: EP1158047-A1 (2 worldwide citation)

The present invention relates to biological substance-immobilized fibers wherein a biological substance is immobilized on a fiber, fibers retaining a biological substance-immobilized gel, and fiber alignments having bundles of the above-described fibers and slices of the same.


6
Yu Fujio, Hashimoto Yoshihiro: Novel circular plasmids derived from the genus rhodococcus.. Nitto Chemical, April 29, 1992: EP0482426-A1 (2 worldwide citation)

The present invention provides an isolated circular plasmid of about 2.6 kb derived from a bacterium belonging to the genus Rhodococcus whose restriction sites comprises two SacI sites, one BamHI site, one PvuI site, one ScaI site, one SphI site and one XhoI site. In addition, the present invention ...


7
AKIYAMA TAKANORI, YU FUJIO, WATANABE FUMIAKI, TAKIKAWA YUYA, SATO EIJI: [fr] HALOHYDRINE EPOXIDASE AMELIOREE, [de] VERBESSERTE HALOHYDRIN-EPOXIDASE, [en] IMPROVED HALOHYDRIN EPOXIDASE. MITSUBISHI RAYON CO, December 9, 2009: EP2130922-A1 (2 worldwide citation)

[en] The present invention provides an industrially useful improved halohydrin epoxidase, a method for producing the same, and a method for producing an epihalohydrin or 4-halo-3-hydroxybutyronitrile using the same. The improved halohydrin epoxidase of the present invention consists of an amino acid ...


8
Watanabe Fumiaki, Yu Fujio: Rhodococcus aspartase protein. Nitto Chemical, December 11, 1998: FR2764303-A1 (1 worldwide citation)

The following are claimed: (1) an aspartase protein having a defined sequence of 477 amino acids given in the specification; (2) DNA encoding the protein of (1); (3) a DNA fragment of about 600 bp that can be amplified with synthetic oligonucleotides having the sequences AACATGAACA CTAACGAGGT and AG ...


9
Watanabe Fumiaki, Yu Fujio: L-aspartic acid preparation. Nitto Chemical, November 27, 1998: FR2763599-A1 (1 worldwide citation)

Production of L-aspartic acid comprises using a Rhodococcus microorganism with aspartase activity and fumaric acid and ammonia as starting materials. The Rhodococcus used is preferably Rhodococcus sp. EA4, R. rhodochrous ATCC 17041, R. rhodochrous ATCC 17895, R. rhodochrous ATCC 19140 or R. rhodochr ...


10
Nakamura Tetsuji, Yu Fujio: Novel amidase gene. Mitsubishi Rayon Co, January 23, 2002: EP1174499-A1 (1 worldwide citation)

The present invention relates to a novel protein having amidase activity that stereoselectively hydrolyzes alpha -amino acid amides and alpha -hydroxy acid amides, and a gene encoding said protein. Namely, the protein is (a) a protein containing an amino acid sequence of SEQ ID NO: 1, or (b) a prote ...



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