1
Yoshihiro Ashihara, Isao Nishizono, Hidetaka Minakawa, Masahisa Okada, Yasusuke Sakurabayashi, Fumio Watanabe, Shin ichi Wakana: Automatic immunological measuring system. Fujirebio, Sughrue Mion Zinn Macpeak and Seas, October 27, 1992: US05158895 (120 worldwide citation)

An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film be ...


2
Yoshihiro Ashihara, Isao Nishizono, Hidetaka Minakawa, Masahisa Okada, Yasusuke Sakurabayashi, Fumio Watanabe, Shin ichi Wakana: Automatic immunological measuring system. January 9, 1996: US05482839 (68 worldwide citation)

An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a ...


3
Masahisa Okada, Yoshihiro Ashihara, Akira Yano, Masao Oishi, Katsuaki Yoshioka, Toshiomi Nakamura: Immunoassay using magnetic particle. Fujirebio, Nippon Paint, Jordan and Hamburg, June 14, 1994: US05320944 (33 worldwide citation)

An immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, The core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer. An antigen or an antibody is bound onto the surface of the coating layer and th ...


4
Motohiro Sasaki, Masahiko Matsukawa, Katsuaki Yoshioka, Makoto Anan, Mitsuo Isomura, Yoshihiro Ashihara, Masahisa Okada: Magnetic particle and immunoassay using the same. Nippon Paint, Fujirebio, Jordan and Hamburg, April 7, 1998: US05736349 (32 worldwide citation)

There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula:


5
Yoshihiro Ashihara, Mitsuo Isomura, Atsuka Sato: Enzyme-labeled immunoassay and device therefor. Fujirebio, Oblon Spivak McClelland Maier & Neustadt P C, April 24, 2001: US06221625 (31 worldwide citation)

An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermin ...


6
Yoshihiro Ashihara, Isao Nishizono, Hidetaka Minakawa, Masahisa Okada, Yasusuke Sakurabayashi, Fumio Watanabe, Shin ichi Wakana: Method of immunoassay measurement. Fujirebio, Cooper & Dunham, March 1, 1994: US05290708 (24 worldwide citation)

An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film be ...


7
Masahisa Okada, Yoshihiro Ashihara, Tadashi Ninomiya, Akira Yano: Chemiluminescence assays using stabilized dioxetane derivatives. Fujirebio, Oblon Spivak McClelland Maier & Neustadt, March 10, 1992: US05094939 (24 worldwide citation)

A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of ...


8
Toshikage Hiraoka, Tetsuji Tanimoto, Yoshihiko Makino, Tadashi Ninomiya, Naofumi Hora, Yoshihiro Ashihara, Yukio Sudo, Toshihiro Mori: Immunoassay element and process and immunoassay. Fuji Photo Film, Fujirebio, McAulay Fisher Nissen Goldberg & Kiel, October 29, 1996: US05569589 (14 worldwide citation)

An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate lay ...


9
Yoshihiro Ashihara, Mitsushi Gotanda, Akira Hasegawa, Yuko Fujiwara: Liquid supply device and analytical test device. Fujirebio, Oblon Spivak McClelland Maier & Neustadt P C, January 25, 2000: US06017494 (13 worldwide citation)

A liquid supply device for transporting a liquid to a predetermined portion includes a liquid container for holding a liquid therein, which has an opening, a sealing sheet member which is impermeable but rupturable and seals the opening of the container, and an absorbent material which is capable of ...


10
Yasushi Kasahara, Hiromasa Suzuki, Yoshihiro Ashihara: Immunoassay method using two immobilized biologically active substances. Fujirebio Kabushiki Kaisha, Burgess Ryan & Wayne, April 15, 1986: US04582792 (9 worldwide citation)

A biologically active composition comprising an immobilized phase comprising an antigen or an antibody, and an immobilized phase comprising an enzyme, an enzyme inhibitor or activator, and a method of measuring antigen or antibody using the same. According to the method of the invention, antigen wit ...