1
Willem P C Stemmer: Methods for in vitro recombination. Affymax Technologies, Townsend & Townsend & Crew, February 25, 1997: US05605793 (496 worldwide citation)

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present inve ...


2
Jeremy Minshull, Willem P C Stemmer: Methods and compositions for cellular and metabolic engineering. Maxygen, Townsend & Townsend & Crew, November 17, 1998: US05837458 (493 worldwide citation)

The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selecti ...


3
Willem P C Stemmer, Andreas Crameri: Methods for generating polynucleotides having desired characteristics by iterative selection and recombination. Affymax Technologies, Townsend & Townsend & Crew, September 22, 1998: US05811238 (490 worldwide citation)

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The p ...


4
Willem P C Stemmer, Andreas Crameri: DNA mutagenesis by random fragmentation and reassembly. Affymax Technologies, Townsend & Townsend & Crew, November 3, 1998: US05830721 (477 worldwide citation)

A method for generating libraries of displayed peptides and/or antibodies (Abs) suitable for affinity interaction screening or phenotypic screening comprising: (i) obtaining selected library members comprising a displayed peptide and/or Ab and the corressponding polynucleotide (PN), or copies of it, ...


5
Willem P C Stemmer: Methods for generating polynucleotides having desired characteristics by iterative selection and recombination. Maxygen, Norman J Kruse, Joe Liebeschuetz, September 12, 2000: US06117679 (241 worldwide citation)

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The p ...


6
Willem P C Stemmer, Robert J Lipshutz: End-complementary polymerase reaction. Glaxo Group, Affymetrix, Townsend & Townsend & Crew, July 27, 1999: US05928905 (200 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof and for assembling large polynucleotides from component polynucleotides, each involving generating concatemers formed by PCR amplification of ov ...


7
Peter J Schatz, Millard G Cull, Jeff F Miller, Willem P C Stemmer: Peptide library and screening method. Affymax Technologies, Townsend and Townsend and Crew, March 12, 1996: US05498530 (193 worldwide citation)

A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also contain a binding site for the DNA binding protein can be used to screen for novel ligands. The scree ...


8
Andreas Crameri, Willem P C Stemmer, Jeremy Minshull, Steven H Bass, Mark Welch, Jon E Ness, Claes Gustafsson, Phillip A Patten: Oligonucleotide mediated nucleic acid recombination. Maxygen, Norman J Kruse, Jonathan Alan Quine, Law Offices of Jonathan Alan Quine, April 23, 2002: US06376246 (188 worldwide citation)

Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.


9
Willem P C Stemmer: Methods of shuffling polynucleotides. Maxygen, Joe Liebeschuetz Esq, Norman Kruse Esq, October 17, 2000: US06132970 (174 worldwide citation)

The invention is directed to methods of shuffling polynucleotide variants. The methods entail conducting a multi-cyclic polynucleotide extension process on partially annealed polynucleotide strands having sequences from the plurality of chosen polynucleotide variants, the polynucleotide strands havi ...


10
Phillip A Patten, Willem P C Stemmer: Methods and compositions for polypeptide engineering. Maxygen, Norman J Kruse, Christopher C Sappenfield, Quine Intellectual Property Law Group, July 1, 2003: US06586182 (126 worldwide citation)

Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed.