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Cabilly Shmuel, Holmes William Evans, Wetzel Ronald Burnell, Heyneker Herbert Louis, Riggs Arthur Dale: Recombinant immunoglobulin preparations, methods for their preparation, dna sequences, expression vectors and recombinant host cells therefor.. Genentech, Hope City, November 14, 1984: EP0125023-A1 (379 worldwide citation)

Recombinant DNA techniques are used to produce both immunoglobulins which are analogous to those normally found in vertebrate systems and to take advantage of these gene modification techniques to construct chimeric or other modified forms.


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Builder Stuart Elliot, Ogez John Robert, Jones Andrew Julian Stuart, Lin Norm Shin Chsiang, Olson Kenneth Charles, Shire Steven Jacob, Pai Rong Chang, Wetzel Ronald Burnell: Methods of purification and reactivation of precipitated heterologous proteins.. Genentech, August 1, 1984: EP0114506-A1 (74 worldwide citation)

A heterologous expression product in host cells is separated from the host cell material by suspending the material in a buffered solution, disrupting the cells and separating the product as refractile material by low speed centrifugation. The refractile material can be dissolved in a strongly denat ...


3
Wetzel Ronald Burnell: Expression of cell wall degrading proteins and host cells harboring dna encoding such protein.. Genentech, September 18, 1985: EP0155189-A2 (21 worldwide citation)

A cell wall lytic enzyme is expressed in recombinant host cells without cell toxicity. The enzyme may be released at a predetermined point by a freeze and thaw cycle. This allows the expressed enzyme to act on the host cell walls and lyse the cells, thereby facilitating recovery of the enzyme or oth ...


4
Wetzel Ronald Burnell: Stable proteins, methods for their construction, plasmids and other dna encoding them, cell cultures harboring such plasmids.. Genentech, September 25, 1985: EP0155832-A2 (10 worldwide citation)

Proteins are mutated in order to introduce disulfide linkages at sites not normally found in the wild type protein. Additional disulfide bonds formed in this fashion stabilize the proteins from inactivation.


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Wetzel Ronald Burnell: Non-naturally occurring zymogens and methods and materials for their construction.. Genentech, September 18, 1985: EP0155190-A2 (1 worldwide citation)

Enzymes are converted from or into reversibly inactive forms, zymogens, by mutating one or more selected wild type amino acid residues, in the enzymes into residues that are capable of chemical inactivation and reactivation. The invention particularly is useful in expressing enzymes normally expecte ...