1
Tammy Burd Mehta, Anne R Kopf Sill, J Wallace Parce, Andrea W Chow, Luc J Bousse, Michael R Knapp, Theo T Nikiforov, Steve Gallagher: Manipulation of microparticles in microfluidic systems. Caliper Technologies, Matthew B Murphy, Donald R McKenna, Quine Intellectual Property Law Group P C, October 14, 2003: US06632655 (384 worldwide citation)

Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and a ...


2
Theo T Nikiforov: Apparatus and methods for sequencing nucleic acids in microfluidic systems. Caliper Technologies, Matthew B Murphy, September 28, 1999: US05958694 (205 worldwide citation)

The present invention is generally directed to microfluidic systems and methods of using such systems in the determination of the nucleotide sequence of target nucleic acid sequences (referred to herein as the "target"). In particular, the present invention provides methods and systems for determini ...


3
J Wallace Parce, Theo T Nikiforov, Tammy Burd Mehta, Anne R Kopf Sill, Andrea W Chow, Michael R Knapp: Sequencing by incorporation. Caliper Technologies, Stacy Landry, Gary Baker, Quine Intellectual Property Law Group P C, September 2, 2003: US06613513 (193 worldwide citation)

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carb ...


4
J Wallace Parce, Theo T Nikiforov, Tammy Burd Mehta, Anne R Kopf Sill, Andrea W Chow, Michael R Knapp: Sequencing by incorporation. Caliper Life Sciences, Donald R McKenna, Ann C Petersen, September 12, 2006: US07105300 (161 worldwide citation)

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carb ...


5
Theo T Nikiforov: Apparatus and methods for sequencing nucleic acids in microfluidic systems. Caliper Technologies, Matthew B Murphy, August 22, 2000: US06107044 (106 worldwide citation)

The present invention is generally directed to microfluidic systems and methods of using such systems in the determination of the nucleotide sequence of target nucleic acid sequences (referred to herein as the "target"). In particular, the present invention provides methods and systems for determini ...


6
Theo T Nikiforov, Sang Jeong: Methods and systems for enhanced fluid transport. Caliper Technologies, Matthew B Murphy, October 12, 1999: US05964995 (105 worldwide citation)

The present invention generally provides methods for enhancing transport and direction of materials in fluidic systems, which systems utilize electroosmotic (E/O) flow systems, to affect that transport and direction. The methods generally comprise providing an effective concentration of at least one ...


7
Theo T Nikiforov: Kinase assays using polycations. Caliper Technologies, Matthew B Murphy, October 29, 2002: US06472141 (72 worldwide citation)

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second r ...


8
Theo T Nikiforov, Michael R Knapp: Method for generating single-stranded DNA molecules. Molecular Tool, Howrey & Simon, May 21, 1996: US05518900 (71 worldwide citation)

A method for generating single-stranded nucleic acid molecules. The molecules contain nuclease resistant modified nucleotides, such that they are resistant to 5'.fwdarw.3' exonucleases.


9
Theo T Nikiforov: Assay methods and system. Caliper Technologies, Matthew B Murphy, September 11, 2001: US06287774 (67 worldwide citation)

Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second r ...


10
H Garrett Wada, Javier A Farinas, Theo T Nikiforov: Methods and systems for monitoring intracellular binding reactions. Caliper Technologies, Matthew B Murphy, April 30, 2002: US06379884 (63 worldwide citation)

Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is co ...