1
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Janet E Hasak, Kevin R Kaster, Albert P Halluin, December 26, 1989: US04889818 (426 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


2
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Lisabeth F Murphy, Kevin R Kaster, January 7, 1992: US05079352 (183 worldwide citation)

Recombinant DNA vectors that encode a thermostable DNA polymerase are useful in the recombinant production of thermostable DNA polymerase. The recombinant thermostable polymerase is preferred for use in the production of DNA in a polymerase chain reaction. Especially useful vectors encode the .about ...


3
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase. Hoffmann La Roche, George W Patricia S Rocha Tramaloni Johnston, Stacey R Sias, April 8, 1997: US05618711 (68 worldwide citation)

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an .about.94 kDa protein especially useful in the DNA amplification procedu ...


4
David H Gelfand, Susanne Stoffel, Randall K Saiki: Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents. Roche Molecular Systems, Stacey R Sias, Douglas A Petry, October 3, 2000: US06127155 (55 worldwide citation)

A purified thermostable nucleic acid polymerase is obtained that has unique characteristics. Preferably the nucleic acid polymerase is DNA polymerase isolated from a Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable nucleic acid polymerase may be n ...


5
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima. Hoffmann La Roche, George W Johnston, Patricia S Rocha Tramaloni, Stacey R Sias, April 29, 1997: US05624833 (53 worldwide citation)

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with prim ...


6
David H Gelfand, Susanne Stoffel: Purified thermostable enzyme. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, October 4, 1994: US05352600 (52 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


7
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Mutated thermostable nucleic acid polymerase enzyme from thermotoga maritima. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, May 30, 1995: US05420029 (49 worldwide citation)

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with prim ...


8
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Recombinant expression vectors and purification methods for thermus thermophilus DNA polymerase. Roche Molecular Systems, George W Johnston, Dennis P Tramaloni, Douglas A Petry, August 4, 1998: US05789224 (36 worldwide citation)

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an .about.94 kDa protein especially useful in the DNA amplification procedu ...


9
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Universal dominant selectable marker cassette. Cetus Corporation, Albert P Halluin, Kate H Murashige, November 15, 1988: US04784949 (24 worldwide citation)

A novel universal dominant selectable marker cassette is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which has been modified and truncated so as to render its use in recombinant vectors more convenient. The modified, truncated sequence (mtAPH- ...


10
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Selectable fusion protein having aminoglycoside phosphotransferase activity. Cetus Corporation, Gregory J Giotta, Albert P Halluin, May 26, 1992: US05116750 (12 worldwide citation)

A novel a selectable fusion protein having aminoglycoside phosphotransferase activity is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which has been modified and truncated so as to render its use in recombinant vectors more convenient. The modi ...