1
Stephen Quake, Marc Unger: Methods and apparatus for analyzing polynucleotide sequences. California Institute of Technology, Proskauer Rose, November 16, 2004: US06818395 (247 worldwide citation)

Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.


2
Stephen Quake, Wayne Volkmuth, Marc Unger: Methods and apparatus for analyzing polynucleotide sequences. California Institute of Technology, Proskauer Rose, June 28, 2005: US06911345 (222 worldwide citation)

The present invention provides an apparatus for analyzing the sequences of polynucleotides. The apparatus comprises (a) flow cell which has at least one microfabricated multilayer elastomeric synthesis channel; and (b) an inlet port and an outlet port. The inlet port and outlet ports are in fluid co ...


3
Stephen Quake, Wayne D Volkmuth: Method and apparatus for analysis and sorting of polynucleotides based on size. California Institute of Technology, Darby & Darby, April 24, 2001: US06221654 (204 worldwide citation)

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting of single polynucleotides, e.g. by size, according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule associ ...


4
Stephen Quake, Ido Braslavsky, Benedict Hebert, Emil Kartalov: Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. California Institute of Technology, Proskauer Rose, November 20, 2007: US07297518 (118 worldwide citation)

The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence microscopy to image polynucleotide molecules at single molecule resolution.


5
Stephen Quake, Wayne D Volkmuth: Methods for analysis and sorting of polynucleotides. California Institute of Technology, Darby & Darby, February 5, 2002: US06344325 (113 worldwide citation)

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting individual polynucleotides, e.g., by size according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule assoc ...


6
Stephen Quake, Hei Mun Christina Fan: Non-invasive fetal genetic screening by digital analysis. The Board of Trustees of the Leland Stanford Junior University, David J Aston, Peters Verny, February 15, 2011: US07888017 (87 worldwide citation)

The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneupl ...


7
Marc Unger, Hou Pu Chou, Todd Thorsen, Axel Scherer, Stephen Quake, Markus Enzelberger, Mark Adams, Carl Hansen: Microfabricated elastomeric valve and pump systems. California Institute of Technology, Townsend and Townsend and Crew, May 15, 2007: US07216671 (86 worldwide citation)

A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a se ...


8
Shulamit Eyal, Stephen Quake: Velocity independent analyte characterization. California Institute of Technology, Townsend and Townsend and Crew, March 16, 2010: US07678547 (84 worldwide citation)

The present invention provides apparatuses for determining velocity independent analyte characteristic parameters and methods for using the same. In one particular aspect, the present invention provides a velocity independent flow cytometry.


9
Stephen Quake, Emil Paskalev Kartalov: Methods and apparatuses for analyzing polynucleotide sequences. Helicos BioSciences, Cooley Godward Kronish, Thomas C Meyers Esq, Konstantin M Linnik Esq, March 10, 2009: US07501245 (58 worldwide citation)

Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.


10
Stephen Quake, Hei Mun Christina Fan: Determination of fetal aneuploidies by massively parallel DNA sequencing. The Board of Trustees of the Leland Stanford Junior University, David J Aston, Peters Verny, August 30, 2011: US08008018 (57 worldwide citation)

The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneupl ...