1
Peter Zaun, Stanley R Bouma, Julian Gordon, John J Kotlarik: Apparatus and method for amplifying and detecting target nucleic acids. Abbott Laboratories, Thomas D Brainard, May 16, 1995: US05415839 (235 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection c ...


2
Stanley R Bouma, Omar S Khalil, Edward K Pabich: Method for detection of nucleic acid using total internal reflectance. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, December 17, 1996: US05585242 (136 worldwide citation)

An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection over the course of the amplification reaction. A method and apparatus for detecting target nucleic acid is also provid ...


3
Stanley R Bouma, Ronald A Coules, Julian Gordon, Eric B Shain, Natalie A Solomon, Peter Zaun: Device and method for amplifying and detecting target nucleic acids. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, July 8, 1997: US05645801 (117 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then sealably mated with a detection c ...


4
Stanley R Bouma, Julian Gordon, Joanell Hoijer, Cynthia Jou, James Rhoads: Method of multiplex ligase chain reaction. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, February 9, 1999: US05869252 (92 worldwide citation)

The invention relates to multiplex ligase chain reaction (LCR). Two or more purative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label ...


5
Natalie A Solomon, Stanley R Bouma: Wide dynamic range nucleic acid detection using an aggregate primer series. Abbott Laboratories, Paul D Yasger, January 12, 1999: US05858732 (67 worldwide citation)

The present invention provides a method of detecting the amount of a target sequence which may be present in a test sample. The method uses an aggregate primer series, which comprises at least two primer sets, in an amplification reaction to detect the relative concentration of a target sequence whi ...


6
Peter Zaun, Stanley R Bouma, Julian Gordon, John J Kotlarik, Natalie A Solomon: Apparatus and method for transfer of a fluid sample. Abbott Laboratories, Paul D Yasger, May 30, 2000: US06068978 (66 worldwide citation)

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a dete ...


7
Thomas G Laffler, Stanley R Bouma, Uwe Spies: Detection and amplification of target nucleic acid sequences. Abbott Laboratories, Thomas D Brainard, Paul D Yasger, January 12, 1999: US05858652 (61 worldwide citation)

A method and kits for amplifying and detecting target nucleic acid sequences in a sample is disclosed. The method employs primers which have reactive pair members linked to them. The reactive pair members can be attached to a solid phase and/or detected by labeled conjugate.


8
Stanley R Bouma, Jeffrey L Joseph, Ronald L Marshall, Thomas G Laffler: Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods. Abbott Laboratories, Paul D Yasger, Thomas D Brainard, January 16, 1996: US05484699 (59 worldwide citation)

Short nucleotide sequences of human papilloma virus useful for the determination of the presence and type of human papilloma virus present in a test sample. The sequences provided can be amplified by polymerase chain reaction or ligase chain reaction. The sequences provided also can be hybridized by ...


9
Edward N Granados, Stanley R Bouma, John J Carrino, Natalie A Solomon: Method and reagent for detecting multiple nucleic acid sequences in a test sample. Abbott Laboratories, Paul D Yasger, September 21, 1999: US05955268 (27 worldwide citation)

Provided herein are methods for detecting multiple target nucleic acid sequences in a test sample. Also provided is a hybridization platform useful for detecting multiple target sequences in a test sample. The hybridization platform comprises a solid support material having a defined pattern of capt ...


10
Stanley R Bouma, Lawrence J Blecka, Philip Miller: Electrophoresis method using vesicles. Abbott Laboratories, Daniel R Curry, April 9, 1991: US05006473 (23 worldwide citation)

The electrophoresis method of the present invention employs a media which contains uniformly dispersed liposomes of phospholipid, or combinations of phospholipid and neutral lipid, which contain chromogenic materials or dye precursers. After electrophoresis of a test sample, the liposomes are lysed ...