1
Seoju Lee, Charles McNemar: Substantially pure histidine-linked protein polymer conjugates. Enzon, Roberts & Mercanti, November 16, 1999: US05985263 (97 worldwide citation)

Substantially pure histidine-linked protein-polymer conjugates and processes for their preparation are disclosed. The processes include contacting a protein with an activated polymer under conditions sufficient to facilitate covalent attachment of at least a portion of the polymer strands on histidi ...


2
Seoju Lee, David C Wylie, Susan V Cannon Carlson: Pegylated interleukin-10. Schering Corporation, May 30, 2006: US07052686 (11 worldwide citation)

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selec ...


3
Susan Cannon Carlson, Andres Frei, Seoju Lee, Roland Mengisen, Marcio Voloch, David C Wylie: Methods for conversion of protein isoforms. Schering Corporation, Donald W Wyatt, August 28, 2001: US06281337 (8 worldwide citation)

The present invention provides methods for isolating adjunct isoforms of proteins and converting them to the desired protein. In preferred embodiments, the present invention contemplates the use of acid solutions or zinc solutions to cleave a chemical group from a desired protein. In further preferr ...


4
Seoju Lee, David C Wylie, Susan Cannon Carlson: Pegylated interleukin-10. Merck Sharp & Dohme Corporation, Carol L Francis, Bozicevic Field & Francis, January 19, 2016: US09238079 (1 worldwide citation)

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selec ...


5
Seoju Lee, David C Wylie, Susan Cannon Carlson: Pegylated interleukin-10. Merck Sharp & Dohme Corporation, Carol L Francis, Bozicevic Field & Francis, April 15, 2014: US08697045 (1 worldwide citation)

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selec ...


6
Anthony O Chong, Seoju Lee, Bhalchandra V Joshi, Brian Bray, Shaoyong Nie, Patrick L Spence, Antoni Kozlowski, Samuel P McManus, Sachin Tipnis, Greg Lavaty, David Swallow, John R Handley, Anthony G Schaefer: Salt form of a multi-arm polymer-drug conjugate. Nektar Therapeutics, Mark A Wilson, Susan T Evans, January 5, 2016: US09226969

Among other aspects, provided herein is a hydrohalide salt of a multi-arm water-soluble polyethylene glycol-drug conjugate, along with related methods of making and using the same. The hydrohalide salt is stably formed, and appears to be more resistant to hydrolytic degradation than the correspondin ...


7
Anthony O Chong, Seoju Lee, Bhalchandra V Joshi, Brian Bray, Shaoyong Nie, Patrick L Spence: Acid salt forms of polymer-drug conjugates and alkoxylation methods. NEKTAR THERAPEUTICS, Mark A Wilson, April 26, 2016: US09320808

Among other aspects, provided herein is a mixed-acid salt of a water-soluble polymer-drug conjugate, along with related methods of making and using the same. The mixed-salt acid salt is stably formed, and appears to be more resistant to hydrolytic degradation than the corresponding predominantly pur ...


8
Seoju Lee, Gary J Vellekamp: Methods for quantifying polymer attachment to a particle. Schering Plough Corporation, Patent Department, May 31, 2007: US20070122800-A1

The present invention relates to improved methods for quantifying the degree of polymer attachment of particles with multiple polymer attachment sites. The disclosed methods are useful for gene therapy, particularly gene therapy using pegylated adenoviral vectors.


9
Seoju Lee, David C Wylie, Susan V Cannon Carlson: Pegylated interleukin-10. Schering Corporation, Dnax Research, Legal Department, December 25, 2008: US20080317707-A1

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selec ...


10
Seoju Lee, David C Wylie, Susan V Cannon Carlson: Pegylated interleukin-10. Schering Plough Corporation, Patent Department, April 18, 2002: US20020044921-A1

Interleukin-10 (IL-10) conjugated via a linker to one or more polyethylene glycol (PEG) molecules at a single amino acid residue of the IL-10, and a method for preparing the same, are provided. The method produces a stable mono-pegylated IL-10, which retains IL-10 activity, where pegylation is selec ...