1
Osamu Hasegawa, Satoshi Aotsuka, Soichiro Takenishi, Hirofumi Uchimiya: Cotton plant gene. Nisshinbo, Jones & Askew, January 2, 2001: US06169174 (80 worldwide citation)

Nucleotide sequences of cotton plant cDNA clones randomly selected are determined. Homology search is performed between the sequences and known gene sequences registered in the data base. Thus a gene, which controls the fiber formation mechanism in cotton plant and which can be used for industrially ...


2
Osamu Hasegawa, Satoshi Aotsuka, Takahisa Hayashi, Yuri Ihara: Cellulose synthase gene. Nisshinbo, Takahisa Hayashi, Jones & Askew, April 18, 2000: US06051407

mRNA was extracted at the stage for cotton plant fibrous cells to accumulate cellulose, and cDNA's complementary thereto were synthesized to construct a cDNA library. Clones of a number of 750 were arbitrarily selected from the library, and they were randomly subjected to sequencing. Those having ho ...


3
Satoshi Aotsuka: Method for producing DNA. Nisshinbo, Knobbe Martens Olson & Bear, January 4, 2005: US06838266

DNA is produced by preparing oligomers having partial sequences selected according to a specific scheme based on a target nucleotide sequence, and performing PCR using two of single strand DNAs base-paired at their 3′ ends as primers and templates to prepare DNA, in a specific manner.


4
Satoshi Aotsuka: Method for producing DNA. Knobbe Martens Olson & Bear, March 21, 2002: US20020034791-A1

DNA is produced by preparing oligomers having partial sequences selected according to a specific scheme based on a target nucleotide sequence, and performing PCR using two of single strand DNAs base-paired at their 3 ends as primers and templates to prepare DNA, in a specific manner.


5
Satoshi Aotsuka: Method for producing DNA. Knobbe Martens Olson & Bear, June 12, 2003: US20030109009-A1

A vector comprising one recognition sequence of a first restriction enzyme of which digestion site exists at a particular position with respect to the recognition sequence and does not exist within the recognition sequence and one recognition sequence of a second restriction enzyme of which digestio ...