1
Robert B Hespell, Herbert A Wyckoff, Bruce S Dien, Rodney J Bothast: Stabilization of pet operon plasmids and ethanol production in bacterial strains lacking lactate dehydrogenase and pyruvate formate lyase activities. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Randall E Deck, John D Fado, August 28, 2001: US06280986 (60 worldwide citation)

Recombinant bacteria are disclosed which are transformed with heterologous DNA coding for alcohol dehydrogenase (adh) and pyruvate decarboxylase (pdc), and which are effective for use in the production of ethanol, but which do not require the presence of antibiotics in the culture medium to maintain ...


2
Badal C Saha, Rodney J Bothast: Thermostable .alpha.-L-arabinofuranosidase from Aureobasidium pullulans. The United States of America represented by the Secretary of Agriculture, M Howard Silversein, Randall E Deck, John D Fado, March 16, 1999: US05882905 (37 worldwide citation)

An .alpha.-L-arabinofuranosidase enzyme which is highly thermostable, and is effective for the hydrolysis of arabinofuranosyl residues from L-arabinose containing polysaccharides and hemicelluloses is disclosed. The enzyme is produced by color variant Aureobasidium pullulans strain NRRL Y-21792. Thi ...


3
Badal C Saha, Rodney J Bothast: Glucose and cellobiose tolerant .beta.-glucosidase from Candida peltata. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Randall E Deck, John D Fado, May 5, 1998: US05747320 (24 worldwide citation)

A new .beta.-glucosidase enzyme which is tolerant to glucose and cellobiose, and which is effective for the hydrolysis of cellobiose to glucose, is disclosed. The enzyme is produced by Candida peltata, strain NRRL Y-21603. This .beta.-glucosidase may be used in conjunction with cellulolytic enzymes ...


4
Cletus P Kurtzman, Rodney J Bothast, James E VanCauwenberge: Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, David G McConnell, Curtis P Ribando, November 16, 1982: US04359534 (14 worldwide citation)

A method is disclosed for converting D-xylose to ethanol relying on the unique ability of the yeast Pachysolen tannophilus to ferment this five-carbon sugar without the use of added enzymes. This process will be particularly useful in the production of ethanolic fuel from plant biomass.


5
Patricia J Slininger, David A Schisler, Rodney J Bothast: Bacteria for the control of fusarium dry rot to potatoes. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, September 3, 1996: US05552315 (11 worldwide citation)

Bacteria which will suppress fungus-induced potato disease under storage conditions have been screened and selected from soil samples. A method for isolating these antagonists, their use in controlling potato disease, and specific isolates which are inhibitory to potato dry rot disease under post-ha ...


6
Nancy N Nichols, Maria J Lopez, Bruce S Dien, Rodney J Bothast: fungus, Culture containing biomass acid hydrolysate and. The United States of America represented by the Secretary of Agriculture, John D Fado, Curtis P Ribando, Lesley Shaw, June 27, 2006: US07067303 (7 worldwide citation)

Agricultural biomass hydrolysate is detoxified by culturing in the presence of the fungus Coniochaeta ligniaria (teleomorph) or its Lecythophora (anamorph) state. This organism is capable of significantly depleting the toxicant levels of furans, particularly furfural and 5-hydroxymethylfurfural. A n ...


7
Patricia J Slininger, James E Vancauwenberge, Rodney J Bothast: Production of 3-hydroxypropionaldehyde from glycerol by Klebsiella pneumoniae. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, Margaret A Connor, October 9, 1990: US04962027 (4 worldwide citation)

A method is disclosed for producing 3-hydroxypropionaldehyde (3-HPA) from glycerol by culturing the bacterium Klebsiella pneumoniae having the identifying characteristics of NRRL B-4011, under aerobic conditions, in an aqueous nutrient medium containing glycerol and a compound that causes 3-HPA to b ...


8
Patricia J Slininger, Karen D Burkhead, David A Schisler, Rodney J Bothast: Biological control of sprouting in potatoes. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, August 22, 2000: US06107247 (2 worldwide citation)

Sprouting in stored potatoes is suppressed with sprout control agents of bacterial origin. These agents are typically applied to the potato surfaces as whole culture broths and they prevent softening and necrosis of the tuber. In a preferred embodiment of the invention, selected isolates also have t ...


9
David A Schisler, Rodney J Bothast, Patricia J Slininger: Bacterial control of Fusarium dry rot of potatoes. The United States of America represented by the Secretary of Agriculture, M Howard Silverstein, Curtis P Ribando, John D Fado, July 21, 1998: US05783411 (2 worldwide citation)

Bacteria which will suppress fungus-induced potato disease under storage conditions have been screened and selected from soil samples. A method for isolating these antagonists, their use in controlling potato disease, and specific isolates which are inhibitory to potato dry rot disease under post-ha ...



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