1
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: Monitoring amplification of DNA during PCR. University of Utah Research Foundation, Barnes & Thornburg, January 16, 2001: US06174670 (363 worldwide citation)

Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Method ...


2
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: Monitoring hybridization during PCR using SYBR™ Green I. University of Utah Research Foundation, Barnes & Thornburg, May 27, 2003: US06569627 (106 worldwide citation)

The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a double-stranded nucleic acid binding dye capable of p ...


3
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: PCR method for nucleic acid quantification utilizing second or third order rate constants. University of Utah Research Foundation, Barnes & Thornburg, May 15, 2001: US06232079 (65 worldwide citation)

The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of am ...


4
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen, David R Hillyard: System and method for fluorescence monitoring. University of Utah Research Foundation, Barnes & Thornburg, July 25, 2006: US07081226 (51 worldwide citation)

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are plac ...


5
Kirk M Ririe, Michael R Newswander, Randy P Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E Jones, Gary Clark Kessler: Self-contained biological analysis. BioFire Diagnostics, Maginot Moore & Beck, March 12, 2013: US08394608 (27 worldwide citation)

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.


6
Carl T Wittwer, Randy P Rasmussen, Marla Lay: Monitoring amplification with fret probes. University of Utah Research Foundation, Barnes & Thornburg, January 9, 2007: US07160998 (12 worldwide citation)

The present invention is directed to kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to kits comprising a first oligonucleotide labeled with a first fluorophore, a second oligonucleotide labeled with a second fluorophore, and components for a ...


7
Karim Tabiti, Gisela Betzl, Richie Soong, Randy P Rasmussen, Deepika Marine Desilva, John G Ward, Hallegh Page Millward: Quantitative multiplex PCR with high dynamic range. Roche Diagnostics Corporation, Idaho Technology, Townsend and Townsend and Crew, Charles M Doyle, October 10, 2006: US07118867 (10 worldwide citation)

The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/μl.


8
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: Annealing curve analysis in PCR. University of Utah Research Foundation, BioFire Diagnostics, Maginot Moore & Beck, January 1, 2013: US08343754 (9 worldwide citation)

The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exch ...


9
David J Eyre, Randy P Rasmussen, Brian E Caplin, Wade R Stevenson, Deepika Marine deSilva: Real-time gene quantification with internal standards. Idaho Technology, University of Utah Research Foundation, Barnes & Thornburg, December 8, 2009: US07630837 (4 worldwide citation)

The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine ...


10
Kirk M Ririe, Michael R Newswander, Randy P Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E Jones, Gary Clark Kessler: Self-contained biological analysis. BioFire Diagnostics, Myers Bigel P A, August 28, 2018: US10058868

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.