1
Kary B Mullis, Henry A Erlich, Norman Arnheim, Glenn T Horn, Randall K Saiki, Stephen J Scharf: Process for amplifying, detecting, and/or-cloning nucleic acid sequences. Cetus Corporation, Janet E Hasak, Albert P Halluin, July 28, 1987: US04683195 (3575 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


2
Kary B Mullis, Henry A Erlich, David H Gelfand, Glenn Horn, Randall K Saiki: Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halloin, October 23, 1990: US04965188 (1379 worldwide citation)

A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary ...


3
Kary B Mullis, Henry A Erlich, Norman Arnheim, Glenn T Horn, Randall K Saiki, Stephen J Scharf: Process for amplifying, detecting, and/or cloning nucleic acid sequences. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halluin, January 24, 1989: US04800159 (1164 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


4
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Homogeneous assay system using the nuclease activity of a nucleic acid polymerase. Hoffman La Roche, George M Gould, Dennis P Tramaloni, Stacev R Sias, May 11, 1993: US05210015 (646 worldwide citation)

The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide frag ...


5
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Janet E Hasak, Kevin R Kaster, Albert P Halluin, December 26, 1989: US04889818 (435 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


6
Randall K Saiki, Henry A Erlich: Method for detection of polymorphic restriction sites and nucleic acid sequences. Cetus Corporation, Janet E Hasak, Albert P Halluin, July 28, 1987: US04683194 (292 worldwide citation)

In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. Th ...


7
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Nucleic acid detection by the 5-3exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, January 30, 1996: US05487972 (271 worldwide citation)

A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is e ...


8
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Reaction mixtures for detection of target nucleic acids. Roche Molecular Systems, George W Johnston, Patricia S Rocha Tramaloni, Stacey R Sias, September 8, 1998: US05804375 (200 worldwide citation)

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorpo ...


9
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Lisabeth F Murphy, Kevin R Kaster, January 7, 1992: US05079352 (186 worldwide citation)

Recombinant DNA vectors that encode a thermostable DNA polymerase are useful in the recombinant production of thermostable DNA polymerase. The recombinant thermostable polymerase is preferred for use in the production of DNA in a polymerase chain reaction. Especially useful vectors encode the .about ...


10
Henry A Erlich, Glenn Horn, Randall K Saiki, Kary B Mullis: Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids. Hoffmann La Roche, George M Gould, Stacey R Sias, Douglas A Petry, November 21, 1995: US05468613 (88 worldwide citation)

Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and ...