1
Marcel B Bally, Nancy L Boman, Pieter R Cullis, Lawrence D Mayer: Liposomal compositions for enhanced retention of bioactive agents. Inex Pharmaceuticals Corporation, University of British of Columbia, Townsend and Townsend and Crew, January 21, 1997: US05595756 (240 worldwide citation)

Liposomal compositions encapsulating bioactive agents and having improved circulation longevity of the agents are disclosed. Such liposomes combine a low pH of the solution in which a bioactive agent is entrapped and a sugar-modified lipid or an amine-bearing lipid, the combination of which enhances ...


2
Pieter R Cullis, Michael J Hope, Marcel B Bally: Extrusion technique for producing unilamellar vesicles. The Liposome Company, Allen Bloom, Catherine Kurtz, Ronald G Ort, April 16, 1991: US05008050 (160 worldwide citation)

A method for reducing the lamellarity of a population of liposomes is provided which comprises repeatedly passing the liposomes under pressure through a filter which has a pore size equal to or less than about 100 nm. In certain embodiments, the method is used to convert a population of previously f ...


3
Jeffery J Wheeler, Marcel B Bally, Yuan Peng Zhang, Dorothy L Reimer, Michael Hope, Pieter R Cullis, Peter Scherrer: Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer. Inex Pharmaceuticals, Townsend and Townsend and Crew, November 2, 1999: US05976567 (158 worldwide citation)

Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic ...


4
Marcel B Bally, Pieter R Cullis, Michael J Hope, Thomas D Madden, Lawrence D Mayer: Encapsulation of antineoplastic agents in liposomes. The Liposome Company, Allen Bloom, Catherine L Kurtz, December 31, 1991: US05077056 (122 worldwide citation)

Methods for encapsulating ionizable antineoplastic agents in liposomes using transmembrane potentials are provided. Trapping efficiencies approaching 100% and rapid loading are readily achieved. Dehydration protocols which allow liposomes to be conveniently used in the administration of antineoplast ...


5
Michael J Hope, Pieter R Cullis: Induction of asymmetry in vesicles. The Liposome Company, Allen Bloom, Catherine L Kurtz, April 20, 1993: US05204112 (105 worldwide citation)

Methods are described for controlling the transbilayer distribution of ionizable lipids and proteins in vesicles. Control of the ion gradient of the exterior bathing medium in relation to that of the interior entrapped aqueous compartment of the vesicles induces migration of ionizable lipids or prot ...


6
Andrew S Janoff, Pieter R Cullis, Marcel B Bally, Michael W Fountain, Richard S Ginsberg, Michael J Hope, Thomas D Madden, Hugh P Schieren, Regina L Jablonski: Dehydrated liposomes. The Liposome Company, Allen Bloom, Catherine L Kurtz, November 14, 1989: US04880635 (98 worldwide citation)

Dehydrated liposomes are prepared by drying liposome preparations under reduced pressure in the presence of one or more protective sugars, e.g., the disaccharides trehalose and sucrose. Preferably, the protective sugars are present at both the inside and outside surfaces of the liposome membranes. F ...


7
John W Holland, Thomas D Madden, Pieter R Cullis: Bilayer stabilizing components and their use in forming programmable fusogenic liposomes. The University of British Columbia, Townsend and Townsend and Crew, March 23, 1999: US05885613 (92 worldwide citation)

The present invention provides a fusogenic liposome comprising a lipid capable of adopting a non-lamellar phase, yet capable of assuming a bilayer structure in the presence of a bilayer stabilizing component; and a bilayer stabilizing component reversibly associated with the lipid to stabilize the l ...


8
Jeffery J Wheeler, Michael Hope, Pieter R Cullis, Marcel B Bally: Methods for encapsulating plasmids in lipid bilayers. Inex Pharmaceuticals, Townsend and Townsend and Crew, November 9, 2004: US06815432 (91 worldwide citation)

Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stabl ...


9
Jeffery J Wheeler, Michael Hope, Pieter R Cullis, Marcel B Bally: Methods for encapsulating plasmids in lipid bilayers. Inex Pharmaceuticals, Townsend and Townsend and Crew, November 9, 1999: US05981501 (88 worldwide citation)

Plasmid-lipid particles which are useful for transfection of cells in vitro or in vivo are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. The particles are typically 65-85 nm, fully encapsulate the plasmid and are serum-stabl ...


10
Helen C Loughrey, Pieter R Cullis, Marcel B Bally, Lewis S Choi: Method for protein-liposome coupling. The Liposome Company, Kenneth B Rubin, March 21, 1995: US05399331 (86 worldwide citation)

This invention relates to a method for synthesizing a substantially pure reactive lipid including, for example, N-[4-(p-maleimidophenyl)-butyryl]phosphatidylethanolamine (MPB-PE) and related compositions. The compositions of the present invention are useful as coupling agents and may be incorporated ...