1
Michael M Harpold, Paul Brust: Assay methods and compositions for detecting and evaluating the intracellular transduction of an extracellular signal. Salk Institute Biotechnology Industrial Associates, Brown Martin Haller & McClain, July 25, 1995: US05436128 (171 worldwide citation)

Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains ...


2
David W Stroman, Paul F Brust, Steven B Ellis, Thomas R Gingeras, Michael M Harpold, Juerg F Tschopp: Regulatory region for heterologous gene expression in yeast. Phillips Petroleum Company, J E Phillips, August 8, 1989: US04855231 (107 worldwide citation)

Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the ...


3
Michael M Harpold, Paul Brust: Assay methods and compositions useful for measuring the transduction of an intracellular signal. The Salk Institute Biotechnology Industrial Associates, Brown Martin Haller & McClain, March 28, 1995: US05401629 (102 worldwide citation)

Recombinant cells are provided which are useful for assaying compounds for their agonist or antagonist activity with respect to ion channels and/or cell surface-localized receptors. In addition, assay methods employing the invention recombinant cells are provided.


4
David W Stroman, Paul F Brust, Steven B Ellis, Thomas R Gingeras, Michael M Harpold, Juerg F Tschopp: Methanol inducible genes obtained from pichia and methods of use. Phillips Petroleum Company, J E Phillips, February 28, 1989: US04808537 (79 worldwide citation)

A method for isolating and cloning methanol inducible genes from Pichia is taught. The regulatory regions are useful for the methanol regulated expression of heterologous genes.


5
David W Stroman, James M Cregg, Michael M Harpold, George T Sperl: Transformation of yeasts of the genus pichia. Phillips Petroleum Company, J E Phillips, November 7, 1989: US04879231 (69 worldwide citation)

Process for transforming yeast strains of the genus Pichia is disclosed. Novel yeast strains of the genus Pichia which can be transformed with recombinant DNA material are also disclosed. In addition, a method for isolating functional genes and other functional DNA sequences from yeast strains of th ...


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Juerg F Tschopp, Michael M Harpold, James M Cregg, Richard G Buckholz: Yeast production of hepatitis B surface antigen. Phillips Petroleum Company, Hal Brent Woodrow, J E Phillips, January 23, 1990: US04895800 (42 worldwide citation)

Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon ...


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Michael M Harpold, Steven B Ellis: DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same. The Salk Institute Biotechnology Industrial Associates, Pretty Schroeder Brueggemann & Clark, November 29, 1994: US05369028 (15 worldwide citation)

Human neuronal nicotinic acetylcholine receptor subunits are described, as are methods for producing cells containing functional receptors employing such subunits. Also described are assay methods for determining the presence of functional HnAChRs in transfected cells, and for determining the agonis ...


10
Steven B Ellis, Mark E Williams, Michael M Harpold, Arnold Schwartz, Jean Sartor, Robert Brenner: Cells expressing calcium channel .alpha.2 subunit-encoding DNA, optionally with a reporter gene for screening assays. Sibia Neurosciences, Stephanie L Brown Martin Haller & McClain Seidman, April 8, 1997: US05618720 (13 worldwide citation)

[DNAs] DNA molecules encoding mammalian calcium channel a.sub.1 and a.sub.2 subunits are provided. The [DNAs] DNA molecules are used to transform host cells which may also contain a reporter gene, the transcription of which is responsive to an ion or molecule capable of entering the cell through a c ...