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G Richard Cathcart, Thomas Brennan Marquez, John A Bridgham, George S Golda, Harry A Guiremand, Marianne Hane, Louis B Hoff, Eric Lachenmeier, Melvyn N Kronick, Douglas H Keith, Paul E Mayrand, Michael L Metzker, William J Mordan, Lincoln J McBride, John Shigeura, Chen Hanson Ting, Norman M Whiteley: Automated molecular biology laboratory. Perkin Elmer Applied Biosystems Division, Donald R Boys, Joseph H Smith, August 22, 1995: US05443791 (318 worldwide citation)

A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for pr ...


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Michael L Metzker, Richard A Gibbs: Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis. Baylor College Of Medicine, Fulbright & Jaworski L, March 17, 1998: US05728529 (55 worldwide citation)

Methods for the use of a class of dyes for improved DNA sequencing by the chain termination method of DNA sequencing, and internal labelling of polynucleotides by enzymatic incorporation of fluorescently-labeled ribonucleotides or deoxyribonucleotides are provided. A new class of dyes, BODIPY.RTM. f ...


3
Michael L Metzker, Richard A Gibbs: Alternative dye-labeled primers for automated DNA sequencing. Baylor College of Medicine, Fulbright & Jaworski L, March 25, 1997: US05614386 (44 worldwide citation)

Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY.RTM. fluorophore, has been described recently. The parent heterocyclic molecule of the BODIPY.RTM. fluorophore is a dipyrrometheneboron difluoride compound which is modified to create a broad ...


4
Michael L Metzker, Richard A Gibbs: Alternative dye-labeled primers for automated DNA sequencing. Baylor College of Medicine, Fulbright & Jaworski, January 19, 1999: US05861287 (39 worldwide citation)

Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY.RTM. fluorophores, has been described recently. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a bro ...


5
Graham B I Scott, Carter Kittrell, Robert F Curl, Michael L Metzker: Pulsed-multiline excitation for color-blind fluorescence detection. Rice University, Baylor College of Medicine, King & Spalding, February 7, 2006: US06995841 (32 worldwide citation)

The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better ...


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Michael L Metzker, Richard A Gibbs: Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds for homogenous amplification/detection assays. Fulbright & Jaworski L, November 30, 1999: US05994063 (20 worldwide citation)

The use of BODIPY.RTM. fluorophores for detecting a target nucleic acid is described. The parent heterocyclic molecule of the BODIPY.RTM. fluorophores is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present inventi ...


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Weidong Wu, Vladislav A Litosh, Brian P Stupi, Michael L Metzker: 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing. LaserGen, Fulbright & Jaworski, March 1, 2011: US07897737 (9 worldwide citation)

Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent ...


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Vladislav A Litosh, Megan N Hersh, Brian P Stupi, Weidong Wu, Michael L Metzker: Nucleotides and nucleosides and methods for their use in DNA sequencing. Lasergen, Fulbright & Jaworski L, April 3, 2012: US08148503 (8 worldwide citation)

The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleava ...


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Weidong Wu, Vladislav A Litosh, Brian P Stupi, Michael L Metzker: 3′-OH unblocked nucleotides and nucleosides, base modified with labels and photocleavable, terminating groups and methods for their use in DNA sequencing. LaserGen, Fulbright & Jaworski, June 21, 2011: US07964352 (7 worldwide citation)

Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent ...


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Weidong Wu, Vladislav A Litosh, Brian P Stupi, Michael L Metzker: 3′OH-unblocked, nucleotides and nucleosides base modified with labels and photocleavable, terminating groups and methods for their use in DNA sequencing. Lasergen, Parker Highlander PLLC, January 29, 2013: US08361727 (5 worldwide citation)

Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent ...