1
Eric B Kmiec, Howard B Gamper, Michael C Rice: Targeted chromosomal genomic alterations with modified single stranded oligonucleotides. University of Delaware, Heller Ehrman White & McAuliffe, Daniel M Becker, August 30, 2005: US06936467 (82 worldwide citation)

Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides. The oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base a ...


2
Eric B Kmiec, Howard B Gamper, Michael C Rice: Targeted chromosomal genomic alterations with modified single stranded oligonucleotides. University of Delaware, McCarter & English, June 5, 2007: US07226785 (77 worldwide citation)

Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base analogs.


3
Eric B Kmiec, Howard B Gamper, Michael C Rice, Michael G Usher: Genomics applications for modified OLIGO nucleotides. University of Delaware, McCarter & English, June 12, 2007: US07229767 (2 worldwide citation)

Methods for the production and use of stable complexes of duplex nucleic acid molecules and oligonucleotides are presented. These complexes can be used for the detection and purification of a known nucleic acid target as well as the manipulation of a defined nucleic acid target sequence.


4
Pamela A Havre, Michael C Rice, William K Holloman, Eric B Kmiec: REC2 kinase. Thomas Jefferson University, Cornell Research Foundation, Valigen, Seidel Gonda Lavorgna & Monaco PC, January 16, 2001: US06174694 (2 worldwide citation)

The invention includes a method of phosphorylating a serine containing substrate by incubating the substrate with ATP and an enzyme that is hsRec2 or muRec2 or a derivative thereof. The natural substrates of the kinase activity of Rec2 are the cell cycle control proteins such as p53 and cyclin E. Th ...


5
Eric B Kmiec, William K Holloman, Michael C Rice, Sheryl T Smith, Zhigang Shu: Mammalian and human REC2. Thomas Jefferson University, Cornell Research Foundation, Drinker Biddle & Reath, June 25, 2002: US06410226 (1 worldwide citation)

The invention concerns mammalian recombinase genes (REC2) and their promoters. Over expression of REC2 in a cell is found to facilitate homologous recombination, particularly homologous recombination using a DNA/RNA chimeric oligonucleotide and to sensitize a cell to the apoptotic effects of irradia ...


6
Pamela A Havre, Michael C Rice, William K Holloman, Eric B Kmiec: Rec 2 kinase. Thomas Jefferson University, Cornell Research Foundation, Valigen, Seidel Gonda Lavorgna & Monaco PC, April 3, 2001: US06210916 (1 worldwide citation)

The invention includes a method of phosphorylating a serine containing substrate by incubating the substrate with ATP and an enzyme that is hsRec2 or muRec2 or a derivative thereof. The natural substrates of the kinase activity of Rec2 are the cell cycle control proteins such as p53 and cyclin E. Th ...


7
Eric B Kmiec, Michael C Rice: Polymorphism detection and separation. University of Delaware, McCarter & English, December 23, 2008: US07468244

Methods and compositions for polymorphism detection and separation. The methods are readily multiplexed, can be adapted to a variety of existing detection systems, and permit target amplification without PCR. The methods permit allelic variants selectively to be isolated, with or without contemporan ...


8
Eric B Kmiec, Michael C Rice, Li Liu: Compositions and methods for enhancing oligonucleotide-mediated gene alteration. University of Delaware, McCarter & English, September 26, 2006: US07112405

Composition and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration in vivo, ex vivo and in vitro are presented. These methods and compositions involve cells and cell-free extracts with altered levels or activities of a protein from the RAD52 epistasis group, the mismatch ...


9
Eric B Kmiec, Howard B Gamper, Michael C Rice: Targeted chromosomal genomic alterations with modified single stranded oligonucleotides. University of Delaware, McCarter & English, August 21, 2007: US07258854

Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides. The oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base a ...


10
Eric B Kmiec, Michael C Rice, Li Liu: Compositions and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration. Mccarter & English, Basil S Krikelis, May 31, 2007: US20070122822-A1

Composition and methods for enhancing oligonucleotide-directed nucleic acid sequence alteration in vivo, ex vivo and in vitro are presented. These methods and compositions involve cells and cell-free extracts with altered levels or activities of a protein from the RAD52 epistasis group, the mismatch ...