1
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof. Affymax Technologies, Kenneth J Nussbacher, Kevin R Kaster, Vern Norviel, September 1, 1992: US05143854 (1389 worldwide citation)

Polypeptide arrays can be synthesized on a substrate by attaching photoremovable groups to the surface of a substrate, exposing selected regions of the substrate to light to activate those regions, attaching an amino acid monomer with a photoremovable group to the activated regions, and repeating th ...


2
Stephen P A Fodor, Michael C Pirrung, J Leighton Read, Lubert Stryer: Array of oligonucleotides on a solid substrate. Affymax Technologies, Townsend and Townsend Khourie and Crew, August 29, 1995: US05445934 (878 worldwide citation)

A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, i ...


3
Stephen P A Fodor, Lubert Stryer, J Leighton Read, Michael C Pirrung: Arrays of materials attached to a substrate. Affymetrix, Vern Norviel, Nancy J DeSantis, Joseph Liebeschuetz, April 28, 1998: US05744305 (870 worldwide citation)

A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reacto ...


4
Stephen P A Fodor, Lubert Stryer, Michael C Pirrung, J Leighton Read: Very large scale immobilized polymer synthesis. Affymax Technologies, Kevin R Kaster, Vern Norviel, June 13, 1995: US05424186 (542 worldwide citation)

A method for synthesizing oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of the substrate without irradiation of a first predefined region of the substrate. The irradiation of a second predefined region of the substrate. The irradiation st ...


5
Stephen P A Fodor, Michael C Pirrung, J Leighton Read, Lubert Stryer: Synthesis and screening of immobilized oligonucleotide arrays. Affymax Technologies, Townsend and Townsend and Crew, April 23, 1996: US05510270 (433 worldwide citation)

A method for synthesizing and screening oligonucleotides on a solid substrate. The method provides for the irradiation of a first predefined region of a substrate comprising immobilized nucleotides on its surface, without irradiation of a second predefined region of the substrate. The irradiation st ...


6
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Large scale photolithographic solid phase synthesis of an array of polymers. Affymax Technologies, Kevin R Kaster, Vern Norviel, April 11, 1995: US05405783 (313 worldwide citation)

A technique for the synthesis of arrays of diverse polymers such as polypeptides and nucleic acids. The technique beneficially utilizes solid-phase chemistry techniques. Preferred embodiments utilize photolabile protecting groups, and photolithography. The technique forms polymers with monomer seque ...


7
Ronald W Barrett, Michael C Pirrung, Lubert Stryer, Christopher P Holmes, Steven A Sundberg: Spatially-addressable immobilization of anti-ligands on surfaces. Affymax Technologies, Kevin R Kaster, Jeffrey K Weaver, Karen B Dow, October 12, 1993: US05252743 (295 worldwide citation)

Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise ...


8
Ronald W Barrett, Michael C Pirrung, Lubert Stryer, Christopher P Holmes, Steven A Sundberg: Spatially-addressable immobilization of anti-ligands on surfaces. Affymax Technologies, Lauren L Stevens, September 19, 1995: US05451683 (185 worldwide citation)

Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise ...


9
Stephen P A Fodor, J Leighton Read, Lubert Stryer, Michael C Pirrung: Polypeptide arrays. Affymetrix, Ropes & Gray, Philip McGarrigle Esq, July 19, 2005: US06919211 (176 worldwide citation)

The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and ...


10
Michael C Pirrung, J Leighton Read, Stephen P A Fodor, Lubert Stryer: Nucleic acid arrays. Affymetrix, Townsend and Townsend and Crew, July 17, 2001: US06261776 (176 worldwide citation)

A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, i ...