1
G Richard Cathcart, Thomas Brennan Marquez, John A Bridgham, George S Golda, Harry A Guiremand, Marianne Hane, Louis B Hoff, Eric Lachenmeier, Melvyn N Kronick, Douglas H Keith, Paul E Mayrand, Michael L Metzker, William J Mordan, Lincoln J McBride, John Shigeura, Chen Hanson Ting, Norman M Whiteley: Automated molecular biology laboratory. Perkin Elmer Applied Biosystems Division, Donald R Boys, Joseph H Smith, August 22, 1995: US05443791 (318 worldwide citation)

A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for pr ...


2
Timothy M Woudenberg, Kevin S Bodner, Charles R Connell, Alan M Ganz, Lincoln J McBride, Paul G Saviano, John Shigeura, David H Tracy, Eugene F Young, Linda G Lee: System for real time detection of nucleic acid amplification products. The Perkin Elmer Corporation Applied Biosystems Division, Wilson Sonsini Goodrich & Rosati, July 27, 1999: US05928907 (205 worldwide citation)

A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicat ...


3
Kenneth J Livak, Lincoln J McBride: Passive internal references for the detection of nucleic acid amplification products. The Perkin Elmer Corporation, Scott R The Perkin Elmer Corporation Bortner, April 7, 1998: US05736333 (102 worldwide citation)

The invention relates to passive internal references for use in quantitating the formation of amplification products in a nucleic amplification reaction. The internal amplification reference molecules of the invention comprise a first and second fluorophore joined together through a backbone connect ...


4
William A Andrus, J William Efcavitch, Lincoln J McBride: Chemical capping by phosphitylation during oligonucleotide synthesis. Applied Biosystems, Joseph H Smith, March 28, 1989: US04816571 (63 worldwide citation)

A method is provided for capping failure sequences in oligonucleotide synthesis by phosphitylation. A phosphite monoester is reacted with the 5' or 3' hydroxyl of the failure sequence between successive condensation steps in a synthesis procedure to form a 5' or 3' phosphite diester with the failure ...


5
Douglas H Keith, Mel N Kronick, Lincoln J McBride, Norman M Whiteley: Labeling by simultaneous ligation and restriction. Applied Biosystems, Cooley Godward Castro Huddleson & Tatum, March 3, 1992: US05093245 (51 worldwide citation)

Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of th ...


6
Mel N Kronick, Douglas H Keith, Lincoln J McBride, Norman M Whiteley, Michael W Hunkapiller: Nucleic acid detection with separation. Applied Biosystems, Cooley Godward, April 25, 2000: US06054266 (6 worldwide citation)

Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nuc ...