11
Michael R Loken, Leon W M M Terstappen: Method for analysis of cellular components of a fluid. Becton Dickinson & Co, Robert M Hallenbeck, September 10, 1991: US05047321 (62 worldwide citation)

A method for multi-parameter analysis of cells in a body fluid sample is described which makes use of a plurality of fluorescence measurements, comprising at least two nucleic acid dyes and at least one fluorescently labelled cell surface marker, and a plurality of light scattering measurements. A k ...


12
Gerald J Dolan, Leon W M M Terstappen: Magnetic devices and sample chambers for examination and manipulation of cells. Immunivest Corporation, John B Dann Dorfman Herrell & Skillman Berryhill, October 24, 2000: US06136182 (58 worldwide citation)

An apparatus for observation of magnetically labeled cells comprises an arrangement of magnets that are configured in confrontation across a horizontal gap. The magnets are further configured to produce a substantially uniform vertically-directed gradient in the gap. A sample vessel containing a flu ...


13
Leon W M M Terstappen, Galla C Rao: Apparatus and methods for capture and analysis of particulate entities. Immunivest Corporation, Dann Dorfman Herrell and Skillman, September 23, 2003: US06623983 (58 worldwide citation)

Apparatus and methods are disclosed which facilitate immobilization of magnetically labelled particulate entities, e.g., cells, preferably in a defined pattern, on a collection surface via binding between specific binding pair members, as an aid to particle analysis.


14
Leon W M M Terstappen: Method for the classification and monitoring of leukemias. Becton Dickinson and Company, Robert M Hallenbeck, August 10, 1993: US05234816 (54 worldwide citation)

A method for classifying and monitoring leukemias is disclosed. The method comprises mixing cells from a patient with a plurality of fluorescently labelled monoclonal antibodies, examining the cells by means of flow cytometry, scoring the percent of positive cells in each quadrant in a two-dimension ...


15
Paul A Liberti, Galla Candra Rao, Leon W M M Terstappen: Increased separation efficiency via controlled aggregation of magnetic nanoparticles. Immunivest Corporation, Dann Dorfman Herrell and Skillman, September 23, 2003: US06623982 (53 worldwide citation)

Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for sig ...


16
Leon W M M Terstappen, Galla Chandra Rao, Jonathan W Uhr, Emilian V Racila, Paul A Liberti: Methods and reagents for the rapid and efficient isolation of circulating cancer cells. Immunivest Corporation, Joseph F Aceto, James L Wilcox, February 19, 2008: US07332288 (48 worldwide citation)

A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater s ...


17
Leon W M M Terstappen, Chia Huei Chen: Multidimensional cell differential analysis. Becton Dickinson and Company, David W Highet, September 11, 2001: US06287791 (45 worldwide citation)

This invention relates to an improved method for the multi-parameter analysis of cells from peripheral blood or bone marrow. The method uses a nucleic acid dye, at least two fluorescently labelled monoclonal antibodies and at least two light scatter parameters to differentiate and discriminate betwe ...


18
Ronald A Mickaels, Richard K Dost, Leon W M M Terstappen, Michael R Loken: Method for data transformation. Becton Dickinson and Company, Robert M Hallenbeck, June 29, 1993: US05224058 (44 worldwide citation)

A method for the non-linear, non-logrithmic transformation of one or more parameters of data gathered by means of flow cytometry is disclosed wherein the method seeks to maximize the resolution between populations of interest for each parameter while maintaining the dynamic range of the data recorde ...


19
Paul A Liberti, Galla Candra Rao, Leon W M M Terstappen: Increased separation efficiency via controlled aggregation of magnetic nanoparticles. Immunivest Corporation, Dann Dorfman Herrell and Skillman, September 16, 2003: US06620627 (43 worldwide citation)

Compositions and methods are disclosed which enhance the microscopic observation and analysis of biological entities such as cells, bacteria and viruses by eliminating interfering magnetic clusters created by naturally occurring aggregators of colloidal magnetic particles. Additionally means for sig ...


20
Ben J H Verwer, Leon W M M Terstappen: Automatic lineage assignment of acute leukemias by flow cytometry. Becton Dickinson and Company, Donna R Fugit, February 25, 1997: US05605805 (41 worldwide citation)

A method for automatic lineage assignment of acute leukemias. Eight four-parameter list mode data files are acquired with a flow cytometer in the following sequence: 1. unstained; 2. isotype controls; 3. CD10 FITC, CD19 PE; 4. CD20 FITC, CD5 PE; 5. CD3 FITC, CD22 PE; 6. CD7 FITC, CD33 PE; 7. HLADR F ...