1
Leon W M M Terstappen, Galla Chandra Rao, Jonathan W Uhr, Emilian V Racila, Paul A Liberti: Methods and reagents for the rapid and efficient isolation of circulating cancer cells. Immunivest Corporation, Board of Regents The University of Texas System, Dann Dorfman Herrell and Skillman, April 2, 2002: US06365362 (162 worldwide citation)

A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater s ...


2
Leon W M M Terstappen, Galla C Rao, Dhanesh I Gohel, Brian P Feeley, Steven Gross, Ellen S Church, Paul A Liberti: Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof. Immunivest Corporation, Dann Dorfman Herrell and Skillman, July 8, 1997: US05646001 (124 worldwide citation)

A method for separation of a mixture of biological entities into at least three distinct, subpopulations. Different antibodies are provided, with each antibody bound to a solid support in a unique manner such that by a manipulation of the physical or chemical environment, the bonds between the antib ...


3
Leon W M M Terstappen, Galla Chandra Rao, Jonathan W Uhr, Emilian V Racila, Paul A Liberti: Methods and reagents for the rapid and efficient isolation of circulating cancer cells. Immunivest Corporation, Dann Dorfman Herrell and Skillman, November 11, 2003: US06645731 (106 worldwide citation)

A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis to detect, enumerate and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood and has a greater s ...


4
Leon W M M Terstappen, Paul A Liberti: Methods for biological substance analysis employing internal magnetic gradients separation and an externally-applied transport force. Immunivest Corporation, Dann Dorfman Herrell and Skillman, January 11, 2000: US06013188 (99 worldwide citation)

A method of qualitative analysis of microscopic biological specimens is disclosed in which the specimens are rendered magnetically responsive by immunosspecific binding with feuromagnetic colloid. The specimens are placed in a vessel having a feuromagnetic capture structure supported on a transparen ...


5
Gerald J Dolan, Leon W M M Terstappen: Magnetic separation apparatus and methods employing an internal magnetic capture gradient and an external transport force. Immunivest Corporation, Dann Dorfman Herrell and Skillman, November 16, 1999: US05985153 (79 worldwide citation)

The present invention relates to apparatus and methods for separating, immobilizing, and quantifying biological substances from within a fluid medium. Biological substances are observed by employing a high internal gradient magnetic capture structure formed within a vessel, in conjunction with an ex ...


6
Leon W M M Terstappen, Paul A Liberti: Quantitative cell analysis methods employing magnetic separation. Immunivest Corporation, Dann Dorfman Herrell and Skillman, November 30, 1999: US05993665 (76 worldwide citation)

A method of quantitative analysis of microscopic biological specimens in a fluid medium is disclosed in which the specimens are rendered magnetically responsive by immunospecific binding with ferromagnetic colloid. A known quantity of magnetically-responsive marker particles are added to the fluid m ...


7
Robert A Levine, Stephen C Wardlaw, Leon W M M Terstappen, Kristen L Manion, Rodolfo R Rodriguez, Adrien P Malick, Subhash Dhanesar, Stephen J Lovell, Alvydas J Ozinskas: Assay of blood or other biologic samples for target analytes. Becton Dickinson and Co, William W Jones, November 10, 1998: US05834217 (69 worldwide citation)

A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as ...


8
Robert A Levine, Stephen C Wardlaw, Leon W M M Terstappen, Kristen L Manion, Rodolfo R Rodriguez, Adrien P Malick, Subhash Dhanesar, Stephen J Lovell, Alvydas J Ozinskas: Assay of blood or other biologic samples for target analytes. Becton Dickinson and Co, William W Jones, July 7, 1998: US05776710 (69 worldwide citation)

A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as ...


9
Robert A Levine, Stephen C Wardlaw, Leon W M M Terstappen, Thomas J Mercolino, Diether J Recktenwald: Apparatus for indirect fluorescent assay of blood samples. Becton Dickinson and Company, William W Jones, August 30, 1994: US05342790 (66 worldwide citation)

A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific ...


10
Leon W M M Terstappen, Michael R Loken, Virendra O Shah: Method for discriminating between intact and damaged cells in a sample. Becton Dickinson and Company, Robert M Hallenbeck, October 15, 1991: US05057413 (64 worldwide citation)

A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in con ...