1
Knazek Richard A, Gullino Pietro M, Dedrick Robert L, Kidwell William R: Cell culture on semi-permeable tubular membranes. The United States of America represented by the Secretary of Health, May 13, 1975: US3883393 (94 worldwide citation)

The growth of cells in vitro on semi-permeable, tube-shaped membranes or capillaries is disclosed. Cells are initially allowed to settle onto the outer surfaces of the membranes in a nutrient medium environment while the membranes are continuously perfused by oxygenated nutrient medium flowing throu ...


2
Knazek Richard A, Kidwell William R: Method for the production of in vitro expanded lymphoid cells for use in adoptive immunotherapy.. Cellco, July 1, 1992: EP0491851-A1

This invention provides improved methods for in vitro culturing of therapeutic quantities of expanded subpopulations of lymphoid cells for use in methods of adoptive immunotherapy. Lymphoid cells, which are derived from a patient, are grown in a hollow cell fiber bioreactor culture system in the pre ...


3
Culver Kenneth W, Knazek Richard A, Blaese R Michael: Sustained and continuous production of high titers of recombinant viral vectors and transduced target cells for use in gene therapy.. Us Commerce, Cellco, October 13, 1993: EP0564539-A1

This invention provides improved methods for producing high titers of recombinant viral vectors by culturing producer cells in a hollow fiber bioreactor. The titer of virus in the extra fiber space (EFS) of the hollow fiber bioreactor is sufficient to transduce target cells at a multiplicity of infe ...


4
Gullino Pietro M, Knazek Richard A, Kidwell William R, Dedrick Robert L: Traduction non-disponible, Cell culture on semi-permeable tubular membranes. Cellco, MARKS & CLERK, April 28, 1981: CA1100067

ABSTRACT OF THE DISCLOSURE The growth of cells in vitro on semi-permeable, tube-shapedmembranes or capillaries is disclosed. Cells are initiallyallowed to settle onto the outer surfaces of the membranes in anutrient medium environment while the membranes are continuouslyperfused by oxygenated nutrie ...


5
Bresler Herbert S, Knazek Richard A: Serum-free production of packaged viral vector. Cellco, GOLDSTEIN Jorge A, September 14, 1995: WO/1995/024467

The application concerns a method for the serum-free production of packaged and infectious viral vectors in an artificial capillary system cartridge. The invention further concerns transducing target cells with the viral vectors. The invention is particularly suitable for producing transduced target ...


6
Knazek Richard A, Kidwell William R: Method for the production of in vitro expanded lymphoid cells for use in adoptive immunotherapy. Cellco, STERN Marvin R, April 4, 1991: WO/1991/004317

This invention provides improved methods for $i(in vitro) culturing of therapeutic quantities of expanded subpopulations of lymphoid cells for use in methods of adoptive immunotherapy. Lymphoid cells, which are derived from a patient, are grown in a hollow cell fiber bioreactor culture system in the ...


7
Knazek Richard A, Kidwell William R, Scott Stephen C, Hanley Matthew W: In vitro cell culture reactor. Cellco Advanced Bioreactors, STERN Marvin R, March 8, 1990: WO/1990/002171

A compact in vitro cell culture reactor (10) drives a liquid nutrient media drawn from a standard cell culture bottle reservoir (16) with a sealed pump (24), through intracapillary space of a cell culture cartridge (11), through a length of silicone rubber tubing (18) coiled in an ascending helix an ...


8
Culver Kenneth W, Knazek Richard A, Blaese R Michael: Sustained and continuous production of high titers of recombinant viral vectors and transduced target cells for use in gene therapy. The United States Of, America Represented By, The Secretary Us Department Of Commerce, Cellco, STERN Marvin R, June 25, 1992: WO/1992/010564

This invention provides improved methods for producing high titers of recombinant viral vectors by culturing producer cells in a hollow fiber bioreactor. The titer of virus in the extra fiber space (EFS) of the hollow fiber bioreactor is sufficient to transduce target cells at a multiplicity of infe ...