1
Lawrence J Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J Aquiles Sanchez: Late-PCR. Brandeis University, Foley and Lardner, April 3, 2007: US07198897 (44 worldwide citation)

A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting tempera ...


2
Lawrence J Wangh, John Rice, J Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn: Primers, probes and methods for nucleic acid amplification. Brandeis University, Foley & Lardner, December 15, 2009: US07632642 (5 worldwide citation)

Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, ...


3
Lawrence J Wangh, John Rice, J Aquiles Sanchez, Kenneth Pierce, Jesse Salk, Arthur Reis, Cristina Hartshorn: Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification. Brandeis University, Foley & Lardner, April 14, 2009: US07517977 (3 worldwide citation)

An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplificati ...


4
Kenneth Pierce, Mark Levey: Composite tender with retractable bimini hard-top. Allen Dyer Doppelt Milbrath & Gilchrist, August 11, 2015: US09102384 (1 worldwide citation)

A lightweight tender comprising a hull constructed from substantially a single piece of composite material and a retractable pillar attached to the hull, wherein the retractable pillar defines a longitudinally extending channel. A retractable carbon fiber bimini cover is pivotingly attached to the r ...


5
Lawrence J Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J Aquiles Sanchez: Late-PCR. Brandeis University, Foley Hoag, October 25, 2016: US09476092

A non-symmetric polymerize chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting tempera ...


6
Lawrence J Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J Aquiles Sanchez: Late-PCR. Brandeis University, Casimir Jones S C, February 5, 2013: US08367325

A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting tempera ...


7
Arthur H Reis Jr, Lawrence J Wangh, Kenneth Pierce: detection assays. Brandeis University, Foley Hoag, June 28, 2016: US09376723

Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various Staphylococcus species and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community ...


8
Arthur H Reis Jr, Lawrence J Wangh, Kenneth Pierce: Single probe, multiple temperature, nucleic acid detection methods, kits, and compositions. Brandeis University, Foley Hoag, November 15, 2016: US09493835

Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where di ...


9
Lawrence J Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J Aquiles Sanchez: Late-pcr. Fish & Richardson PC, March 18, 2004: US20040053254-A1

A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting tempera ...


10
Lawrence Wangh, Kenneth Pierce, Cristina Hartshorn, John Rice, J Aquiles Sanchez: Detection of nucleic acids. Brandeis University, Lahive & Cockfield, January 30, 2003: US20030022231-A1

Disclosed are compositions, methods, and kits useful for the detection of the presence and/or quantity of one or more chromosomes from single cells, groups of cells, or subcellular compartments. Provided is a lysis buffer for the preparation of substantially accessible nucleic acid molecules from a ...