1
Bachmann Bernd Dr, Thierbach Georg Dr, Kalinowski Joern, Puehler Alfred Prof Dr: Process for the fermentative production of l-lysine.. Degussa, September 19, 1990: EP0387527-A1 (17 worldwide citation)

A process for the preparation of L-lysine in which recombinant DNA which consists of a DNA fragment which has a genetic sequence coding for the production of proteins which lead to an aspartyl- beta -semialdehyde dehydrogenase (asd) activity or to the deregulation of aspartate kinase (lysC), which s ...


2
Kassing Friedrich, Kalinowski Joern, Arnold Walter, Winterfeldt Andrea, Puehler Alfred Prof, Kautz Petra Sabine, Thierbach Georg Dr: Method for the site-specific mutagenesis of dna and development of plasmid vectors.. Degussa, July 4, 1990: EP0375889-A2 (9 worldwide citation)

The invention relates to a method for the site-specific mutagenesis of DNA at the restriction cleavage sites using hydroxylamine and to the construction of plasmid vectors with cloned resistance genes from C.xerosis and to plasmids which have been mutated by the method and have unique restriction cl ...


3
Dusch Nicole Dr, Kalinowski Joern Dr, Puehler Alfred Prof Dr: Method for the fermentative production of d- pantothenic acid by amplification of the pand gene of microorganisms. Degussa, June 7, 2000: EP1006192-A2 (8 worldwide citation)

Recombinant DNA (I), comprising a nucleotide sequence (Ia) encoding aspartate-1-decarboxylase, present in microorganisms, especially Corynebacterium and Escherichia, is new. Independent claims are also included for the following: (1) microorganisms (II), especially of the genus Corynebacterium or Es ...


4
Kassing Friedrich, Schaefer Andreas, Kalinowski Joern Dr, Puehler Alfred Prof Dr: Transposon, test vectors containing the transposon and method for mutagenesis.. Degussa, September 11, 1991: EP0445385-A2 (7 worldwide citation)

The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilisable, non-self-transferable vectors which contain this transposon, and to methods for the mutagenesis of Gram-positive bacteria by transfer of these vectors and ge ...


5
Elischewski Frank Dr, Kalinowski Joern Dr, Puehler Alfred Prof Dr, Dusch Nikole Dr, Dohmen Juergen Dr, Farwick Mike Dr, Thierbach Georg Dr: Process for the reduction of pantothenic acid by amplification of nucleotide sequences coding for ketopantoate-reductase. Degussa, May 17, 2000: EP1001027-A2 (7 worldwide citation)

Production, and improvement, of panthothenic acid (I)-producing microorganisms by amplifying (particularly overexpressing) sequences (I) that encode ketopanthoate reductase (KPR), specifically the panE gene, either individually or together. Optionally the ilvC gene is also amplified. Independent cla ...


6
Nicole Dusch, Kalinowski Joern, Puehler Alfred: Replicable dna, transformed microorganism, plasmid vector, and production of d-pantothenic acid. Degussa Huels, August 22, 2000: JP2000-228990 (7 worldwide citation)

PROBLEM TO BE SOLVED: To obtain a new replicable DNA which contains a base sequence coding for aspartate-1-decarboxylase, is replicable in a microorganism such as corynebacteria and escherichia, and can be used for producing pantothenic acid by fermentation. SOLUTION: This is a new replicable DNA wh ...


7
Moeckel Bettina Dr, Weissenborn Anke, Pfefferle Walter Dr, Puehler Alfred Prof, Kalinowski Joern Dr, Bathe Brigitte Dr, Dusch Nicole Dr: Nucleotide sequence encoding corynebacterium poxb-gene and its use in the preparation of l-lysine. Degussa, May 2, 2001: EP1096013-A2 (6 worldwide citation)

Isolated polynucleotide (I) comprises: (a) a sequence that is at least 70% identical to a sequence that encodes a polypeptide comprising the fully defined 579 amino acid sequence (S2) given in the specification; (b) encodes a protein at least 70% identical with (S2); (c) a sequence complementary to ...


8
Dusch Nicole, Bathe Brigitte, Kalinowski Joern, Puehler Alfred, Moeckel Bettina, Weissenborn Anke, Pfefferle Walter: New nucleotide sequence encoding poxb-gene. Degussa Huels, June 19, 2001: JP2001-161386 (6 worldwide citation)

PROBLEM TO BE SOLVED: To provide a new method for producing an amino acid, especially L-lysine by an improved fermentation.SOLUTION: The new nucleotide sequence is an isolated polynucleotide containing a polynucleotide sequence selected from the group consisting of a) a polynucleotide identical at l ...


9
Moeckel Bettina, Weissenborn Anke, Pfefferle Walter, Marx Achim, Puehler Alfred, Kalinowski Joern, Bathe Brigitte, Dusch Nicole: Nucleotide sequence encoding the zwa2 protein. Degussa, June 13, 2001: EP1106693-A1 (5 worldwide citation)

Isolated polynucleotide (I) which is a zwa2 gene from Corynebacterium glutamicum, is new. Isolated polynucleotide (I) contains a sequence that is: (i) at least 70% identical with a sequence that encodes a 385 amino acid (aa) sequence (II), given in the specification; (ii) encodes a polypeptide at le ...


10
Rieping Mechthild Dr, Thierbach Georg Dr, Pfefferle Walter Dr, Dusch Nicole Dr, Kalinowski Joern Dr, Puehler Alfred Prof Dr: Method for the fermantative production of d-pantothenic acid using enterobacteriaceae strains. Degussa, June 7, 2000: EP1006193-A2 (2 worldwide citation)

A method (I) for the preparation of D-pantothenic acid (II), comprising the fermentation of D-pantothenic acid-producing microorganisms of the Enterobacteriacae family, is new. A method (I) for the preparation of D-pantothenic acid (II), comprising the fermentation of D-pantothenic acid-producing mi ...



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