1
Frances H Arnold, Zhixin Shao, Joseph A Affholter, Huimin H Zhao, Lorraine J Giver: Recombination of polynucleotide sequences using random or defined primers. California Institute of Technology, Townsend & Townsend & Crew, November 28, 2000: US06153410 (87 worldwide citation)

A method for in vitro mutagenesis and recombination of polynucleotide sequences based on polymerase-catalyzed extension of primer oligonucleotides is disclosed. The method involves priming template polynucleotide(s) with random-sequences or defined-sequence primers to generate a pool of short DNA fr ...


2
Gilman A Hill, Joseph A Affholter: Integrated in situ retorting and refining of oil shale. Edwin H Crabtree, Ramon L Pizarro, July 19, 2011: US07980312 (79 worldwide citation)

A method and system for producing hydrocarbons in situ from an oil shale, fixed bed, hydrocarbon formation disposed below a ground surface. The hydrocarbon formation having an upper A-Groove higher permeability aquifer zone disposed above a R-rated, kerogen rich, lower permeability zone and a lower ...


3
Frances H Arnold, Zhixin Shao, Joseph A Affholter, Huimin Zhao, Lorraine J Giver: Recombination of polynucleotide sequences using random or defined primers. California Institute of Technology, Townsend and Townsend and Crew, January 23, 2001: US06177263 (62 worldwide citation)

A method for in vitro mutagenesis and recombination of polynucleotide sequences based on polymerase-catalyzed extension of primer oligonucleotides is disclosed. The method involves priming template polynucleotide(s) with random-sequences or defined-sequence primers to generate a pool of short DNA fr ...


4
Gilman A Hill, Joseph A Affholter: Integrated in situ retorting and refining of oil shale. Edwin H Crabtree, Ramon L Pizarro, September 11, 2012: US08261823 (60 worldwide citation)

A system and method for producing hydrocarbons in situ from an oil shale, fixed bed hydrocarbon formation disposed below a ground surface and having a naturally occurring, water-flow leached, higher permeability zone next to a lower permeability zone. The system includes at least one injection openi ...


5
Stephen delCardayre, Matthew Tobin, Willem P C Stemmer, Jon E Ness, Jeremy Minshull, Phillip Patten, Venkiteswatan Subramanian, Linda A Castle, Claus M Krebber, Steve Bass, Ying Xin Zhang, Tony Cox, Gjalt Huisman, Ling Yuan, Joseph A Affholter: Evolution of whole cells and organisms by recursive sequence recombination. Maxygen, Norman J Kruse, Joanne R Petithory, December 12, 2006: US07148054 (27 worldwide citation)

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/o ...


6
Peter S Mezes, Ruth A Richard, Joseph A Affholter, Nicolas J Kotite: Dimer and multimer forms of single chain polypeptides. The Dow Chemical Company, Karen L Kimble, Mark S Scott, June 6, 2000: US06071515 (23 worldwide citation)

The present invention discloses novel proteins which are dimers and multimers of single chain polypeptides. The single chain polypeptides having two domains derived from the immunoglobulin superfamily which are joined by a peptide linker. The dimers and multimers being formed by non-covalent linking ...


7
Stephen delCardayre, Matthew B Tobin, Willem P C Stemmer, Jon E Ness, Jeremy S Minshull, Phillip A Patten, Venkiteswatan Mani Subramanian, Linda A Castle, Claus M Krebber, Steven H Bass, Ying Xin Zhang, Anthony R Cox, Gjalt W Huisman, Ling Yuan, Joseph A Affholter: Evolution of whole cells and organisms by recursive sequence recombination. Maxygen, December 8, 2009: US07629170 (22 worldwide citation)

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/ ...


8
Joseph A Affholter, S Christopher Davis, Sergey A Selifonov: DNA shuffling of monooxygenase genes for production of industrial chemicals. Maxygen, Donald J Pochopien, Sharon M Fujita, Norman J Kruse, August 12, 2003: US06605430 (21 worldwide citation)

This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as &agr;-hydroxycarboxylic acids, and aryl- and alkyl-hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectiv ...


9
Peter S Mezes, Ruth A Richard, Joseph A Affholter, Nicolas J Kotite: Dimer and multimer forms of single chain polypeptides. The Dow Chemical Company, Mark S Scott, Karen L Kimble, December 11, 2001: US06329507 (15 worldwide citation)

The present invention discloses novel proteins which are dimers and multimers of single chain polypeptides. The single chain polypeptides having two domains derived from the immunoglobulin superfamily which are joined by a peptide linker. The dimers and multimers being formed by non-covalent linking ...


10
Steven H Bass, S Christopher Davis, Phillip A Patten, Matthew Tobin, Jeremy Minshull, Mark Welch, Claes Gustafsson, Brian Carr, Stephane Jenne, Sun Ai Raillard, Andreas Crameri, Willem P C Stemmer, Robin Emig, Pascal Longchamp, Stanley Goldman, Lorraine J Giver, Joseph A Affholter: Integrated system for diversity generation and screening. Maxygen, Sharon M Fujita, Jonathan A Quine, Norman J Kruse, December 9, 2008: US07462469 (7 worldwide citation)

Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.