1
Philip L Felgner, Jon A Wolff, Gary H Rhodes, Robert W Malone, Dennis A Carson: Delivery of exogenous DNA sequences in a mammal. VICAL Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 3, 1996: US05580859 (762 worldwide citation)

Polynucleotide sequences, comprising DNA and RNA molecules can be directly administered, for example by injection, to tissues, such as muscle, and expressed as a protein, polypeptide or polypeptide. The polynucleotides can be contained within liposomes or the polynucleotides can free from associatio ...


2
Philip L Felgner, Jon A Wolff, Gary H Rhodes, Robert W Malone, Dennis A Carson: Induction of a protective immune response in a mammal by injecting a DNA sequence. Vical Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 31, 1996: US05589466 (546 worldwide citation)

A method for delivering an isolated polynucleotide such as DNA or RNA, to the interior of a cell in a mammal comprising the injection of an isolated polynucleotide into a muscle of the mammal where the polynucleotide is taken up by the cells of the muscle and exerts a therapeutic effect on the mamma ...


3
Jon A Wolff, David J Duke, Philip L Felgner: Expression of exogenous polynucleotide sequences cardiac muscle of a mammal. Vical Incorporated, Wisconsin Alumni Research Foundation, Knobbe Martens Olson & Bear, December 2, 1997: US05693622 (172 worldwide citation)

The present invention provides a method for delivering a pharmaceutical polypeptide to the interior of a cardiac cell of a vertebrate in vivo, comprising the step of introducing a preparation comprising a pharmaceutically acceptable injectable carrier and naked polynucleotide operatively coding for ...


4
Jon A Wolff, Jeffery Fritz, Vladimir Budker, James Hagstrom: Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein. Mirus Corporation, Mark K Johnson, April 28, 1998: US05744335 (60 worldwide citation)

The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are ca ...


5
Jon A Wolff, Vladimir Budker, Stuart J Knechtle: Process of delivering naked DNA into a hepatocyte via bile duct. Mirus, Mark K Johnson, July 24, 2001: US06265387 (59 worldwide citation)

A process for delivering a polynucleotide to a parenchymal cell in a mammal by injecting the polynucleotide into a blood vessel connected to the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell and functionally expressed to thera ...


6
Jon A Wolff, Vladimir Budker, Vladimir Gurevich: Amphipathic, micellar delivery systems for biologically active polyions. Dressler Rockey Milnamow & Katz, June 3, 1997: US05635487 (40 worldwide citation)

The present invention provides a composition comprising a population of micelles wherein each micelle comprises at least one amphipathic compound layer that surrounds a non-aqueous core that contains a polyion. Also provided are a method of preparing such a composition and the uses of such compositi ...


7
Sean D Monahan, Jon A Wolff, Paul M Slattum, James E Hagstrom, Vladimir G Budker: Reverse micelles for delivery of nucleic acids. Mirus Corporation, Mark K Johnson, August 6, 2002: US06429200 (38 worldwide citation)

A complex is described for delivery to a cell comprising inserting a nucleic acid into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery. Other molecules are used to interact with the nucleic acid—micelle complex to further enhance delivery such ...


8
Jon A Wolff, James E Hagstrom, Vladimir G Budker, Vladimir S Trubetskoy: Polyampholytes for delivering polyions to a cell. Mirus Corporation, Mark K Johnson, May 7, 2002: US06383811 (37 worldwide citation)

An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further d ...


9
Jon A Wolff, James E Hagstrom, Sean D Monahan, Vladimir Budker, David B Rozema, Paul M Slattum: Compositions and methods for drug delivery using amphiphile binding molecules. Mirus Corporation, Mark K Johnson, May 25, 2004: US06740643 (35 worldwide citation)

The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.


10
Fred H Gage, Theodore Friedmann, Michael B Rosenberg, Jon A Wolff, Malcolm Schinstine, Michael D Kawaja, Jasodhara Ray: Method of grafting genetically modified cells to treat defects, disease or damage of the central nervous system. The Regents of the University of California, Merchant Gould Smith Edell Welter and Schmidt, July 22, 1997: US05650148 (34 worldwide citation)

Methods of genetically modifying donor cells by gene transfer for grafting into the central nervous system to treat defective, diseased or damaged cells are disclosed. The modified donor cells produce functional molecules that effect the recovery or improved function of cells in the CNS. Methods and ...