1
G Richard Cathcart, Thomas Brennan Marquez, John A Bridgham, George S Golda, Harry A Guiremand, Marianne Hane, Louis B Hoff, Eric Lachenmeier, Melvyn N Kronick, Douglas H Keith, Paul E Mayrand, Michael L Metzker, William J Mordan, Lincoln J McBride, John Shigeura, Chen Hanson Ting, Norman M Whiteley: Automated molecular biology laboratory. Perkin Elmer Applied Biosystems Division, Donald R Boys, Joseph H Smith, August 22, 1995: US05443791 (318 worldwide citation)

A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for pr ...


2
Timothy M Woudenberg, Kevin S Bodner, Charles R Connell, Alan M Ganz, Lincoln J McBride, Paul G Saviano, John Shigeura, David H Tracy, Eugene F Young, Linda G Lee: System for real time detection of nucleic acid amplification products. The Perkin Elmer Corporation Applied Biosystems Division, Wilson Sonsini Goodrich & Rosati, July 27, 1999: US05928907 (205 worldwide citation)

A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicat ...


3
Timothy M Woudenberg, Kevin S Bodner, Charles R Connell, John Shigeura, David H Tracy, Eugene F Young: Apparatus and method for detecting nucleic acid amplification products. Perkin Elmer Corporation Applied Biosystems Division, David J Wilson Sonsini Goodrich & Rosati Weitz, January 18, 2000: US06015674 (107 worldwide citation)

An apparatus is provided which includes a sample holder for holding a reaction chamber which includes an optical interface, a fiber optic cable for delivering an excitation beam to a sample housed within the reaction chamber and for receiving light emitted by the sample, and a lens co-axially dispos ...


4
Zbigniew T Bryning, John Shigeura: Microfluidic devices, methods, and systems. Applera Corporation, Kilyk & Bowersox P L L C, April 3, 2007: US07198759 (57 worldwide citation)

Microfluidic assemblies, systems, and methods are provided for manipulating fluid samples. Assemblies include an elastically deformable cover layer and a less elastically deformable substrate. The methods include deforming the substrate through the cover layer so that when the cover layer rebounds a ...


5
Larry J Carson, Joel R Dufresne, Patrick R Fleming, Michael C Lea, Nicholas A Lee, John Shigeura: Analytical device with lightguide Illumination of capillary and microgrooves arrays. 3M Innovative Properties Company, Applera Corporation, March 13, 2007: US07189361 (52 worldwide citation)

An analytical cell including a lightguide with a plurality of conduits filled with a migration medium. The medium, the lightguide and a surrounding medium have refractive indices selected such that light entering the lightguide is internally reflected within the lightguide to provide substantially u ...


6
Douglas H Smith, John Shigeura, Timothy M Woudenberg: Apparatus for a fluid impingement thermal cycler. The Perkin Elmer Corporation, Daniel B Dehlinger & Associates Curtis, August 24, 1999: US05942432 (40 worldwide citation)

Apparatus are disclosed that thermally cycles samples between at least two temperatures. These apparatus operate by impinging fluid jets onto the outer walls of a sample containing region. Because the impinging fluid jets provide a high heat transfer coefficient between the jet and the sample contai ...


7
Eric S Nordman, Richard T Reel, John Shigeura: Time-delay integration in electrophoretic detection systems. Applera Corporation, Kilyk & Bowersox P L L C, February 15, 2005: US06856390 (32 worldwide citation)

An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted f ...


8
Howard Gregg King, John Shigeura, Eric S Nordman, Sean Matthew Desmond: Sample handling system for a multi-channel capillary electrophoresis device. The Perkin Elmer Corporation, Paul D Grossman, October 17, 2000: US06132582 (31 worldwide citation)

A sample handling system in a multi-channel capillary electrophoresis apparatus is disclosed. The sample handling system includes a work surface for supporting a plurality of samples located at a plurality of work surface coordinates and a sample loading assembly comprising a plurality of loading we ...


9
John Shigeura: Non-contact radiant heating and temperature sensing device for a chemical reaction chamber. Applera Corporation, December 21, 2004: US06833536 (26 worldwide citation)

An apparatus and methods are provided for heating and sensing the temperature of a chemical reaction chamber without direct physical contact between a heating device and the reaction chamber, or between a temperature sensor and the reaction chamber. A plurality of chemical reaction chambers can simu ...


10
John Shigeura, John A Bridgham, Louis B Hoff, P Eric Mayrand: Horizontal polyacrylamide gel electrophoresis. Applied Biosystems, Donald R Boys, Joseph H Smith, August 16, 1994: US05338426 (20 worldwide citation)

An electrophoresis apparatus has a gel film cast between two plates and buffer reservoirs at each end of the film with electrodes connectable to an external power supply for providing electromotive force for driving electrophoresis. The reservoirs are configured to wet the ends of the gel film and s ...