1
Paul N Schnipelsky, Leonard J Seaberg, Charles C Hinckley, Jeffrey A Wellman, William H Donish, John B Findlay: Containment cuvette for PCR and method of use. Eastman Kodak Company, Dana M Schmidt, July 20, 1993: US05229297 (384 worldwide citation)

A cuvette and a method of use which prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments in the cuve ...


2
Paul H D Chen, John B Findlay, Susan M Atwood, Lynn Bergmeyer: Nucleic acid material amplification and detection without washing. Eastman Kodak Company, Dana M Schmidt, June 6, 1995: US05422271 (239 worldwide citation)

A device and method are disclosed for amplifying and detecting nucleic acid material. The device and method use a label and signalling material responsive to the label to produce a detectable signal. A surprising result of the method and device is that at least one of the wash steps heretofore requi ...


3
John W Backus, Tobias E Ekeze, Jerome C Swartz, Richard C Sutton, Ignazio S Ponticello, JoAnne H Kerschner, John B Findlay: Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same. Johnson & Johnson Clinical Diagnostics, December 10, 1996: US05582988 (54 worldwide citation)

Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nuc ...


4
John B Findlay, Janice M Mayer, Marlene M King, Fred T Oakes, Chu an Chang, Corey H Levenson: Nucleic acid test article and its use to detect a predetermined nucleic acid. Johnson & Johnson Clinical Diagnostics, May 7, 1996: US05514550 (46 worldwide citation)

A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble ...


5
Richard C Sutton, Susan J Danielson, John B Findlay, Fred T Oakes, Marsha D B Oenick, Ignazio S Ponticello, Harold C Warren III: Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use. Eastman Kodak Company, James L Tucker, September 15, 1992: US05147777 (36 worldwide citation)

Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carbo ...


6
John B Findlay, Lynn Bergmeyer: Diagnostic and amplification methods using primers having thymine at 3 end to overcome primer-target mismatch at the 3 end. Eastman Kodak Company, J Lanny Tucker, March 23, 1993: US05196305 (23 worldwide citation)

Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3' end of the primer. Such a mismatch is overcom ...


7
Susan M Atwood, Thomas J Cummins, John B Findlay: Diagnostic compositions, elements, methods and test kits for amplification and detection of retroviral DNA using primers having matched melting temperatures. Eastman Kodak Company, James L Tucker, April 4, 1995: US05403707 (17 worldwide citation)

An aqueous composition containing primers for opposing strands of a target retroviral DNA (such as HIV-I DNA) can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of that target DNA and one or more additional target DNA's. The primers for ...


8
Harold C Warren III, John B Findlay, Marlene M King: Nucleic acid probe, test kit and diagnostic and purification methods. Eastman Kodak Company, J Lanny Tucker, July 12, 1994: US05328825 (14 worldwide citation)

An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substanti ...


9
Theodore W Esders, Shirley Y Lynn, John B Findlay, Richard M Schubert: Composition, analytical element and method for the quantification of creatine kinase. Eastman Kodak Company, J Lanny Tucker, October 15, 1985: US04547461 (12 worldwide citation)

An enzymatic method for the analytical determination of creatine kinase in an aqueous liquid such as blood serum is described. The determination is made by measuring an optical density change using the reagents creatine phosphate, adenosine diphosphate, glycerol, glycerol kinase, .alpha.-glycerophos ...


10
John B Findlay, Annie L Wu, Gary E Norton: Determination of total creatine kinase or an isoenzyme with a multilayer analytical element. Eastman Kodak Company, J Lanny Tucker, December 15, 1987: US04713327 (11 worldwide citation)

A multilayer analytical element for the determination of creatine kinase comprises a support having thereon, in order and in fluid contact, a registration layer and an isotropically porous spreading layer. The registration layer contains a binder material at a coverage of at least about 8 g/m.sup.2, ...