1
James M Cregg: Autonomous replication sequences for yeast strains of the genus pichia. Phillips Petroleum Company, J E Phillips, June 6, 1989: US04837148 (180 worldwide citation)

Novel autonomous replication sequences which are capable of maintaining plasmids as extrachromosomal elements in host strains of the genus Pichia are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producin ...


2
James M Cregg, Kevin J Barringer: Pichia transformation. Phillips Petroleum Company, J E Phillips, May 29, 1990: US04929555 (160 worldwide citation)

The present invention provides a method for making whole cells of methylotrophic species of genus Pichia competent for transformation by DNA and a method for transforming with DNA whole cells of such species.


3
James M Cregg: Site selective genomic modification of yeast of the genus pichia. Phillips Petroleum Company, J E Phillips, November 21, 1989: US04882279 (119 worldwide citation)

Method for the site specific genomic modification of yeasts of the genus Pichia and novel DNA sequences useful therefor are provided. Pichia is transformed with a serially arranged linear DNA fragment comprising first and second insertable DNA fragments, which flank a marker gene. The insertable DNA ...


4
David W Stroman, James M Cregg, Michael M Harpold, George T Sperl: Transformation of yeasts of the genus pichia. Phillips Petroleum Company, J E Phillips, November 7, 1989: US04879231 (69 worldwide citation)

Process for transforming yeast strains of the genus Pichia is disclosed. Novel yeast strains of the genus Pichia which can be transformed with recombinant DNA material are also disclosed. In addition, a method for isolating functional genes and other functional DNA sequences from yeast strains of th ...


5
James M Cregg, George T Sperl: Pichia pastoris argininosuccinate lyase gene and uses thereof. Phillips Petroleum Company, J E Phillips, April 4, 1989: US04818700 (58 worldwide citation)

Novel DNA sequences which code for the production of the Pichia protein argininosuccinate lyase are provided. Novel constructs including these sequences, as well as organisms transformed therewith are provided. In addition, novel strains of Pichia defective in argninosuccinate lyase activity are als ...


6
James M Cregg: Genes from pichia histidine pathway and uses thereof. Phillips Petroleum Company, J E Phillips, December 5, 1989: US04885242 (52 worldwide citation)

Novel DNA sequences which code for the production of Pichia histidinol dehydrogenase, phosphoribosyl-ATP-cyclohydrase and phosphoribosyl-ATP-pyrophosphohydratase are provided. Novel constructs including these sequences, as well as transformed organisms therewith are provided. A method for isolation ...


7
Juerg F Tschopp, Michael M Harpold, James M Cregg, Richard G Buckholz: Yeast production of hepatitis B surface antigen. Phillips Petroleum Company, Hal Brent Woodrow, J E Phillips, January 23, 1990: US04895800 (42 worldwide citation)

Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon ...


8
James M Cregg, Mary E Digan: Strains of pichia pastoris created by interlocus recombination. Phillips Petroleum Company, J E Phillips, June 16, 1992: US05122465 (39 worldwide citation)

A process for the regeneration of a selectable phenotype at a high frequency by interlocus recombination, in yeast cultures transformed with recombinant DNA constructs comprised of a gene whose product complements the selectable phenotype to be regenerated is disclosed. A sequence of steps is involv ...


9
James M Cregg: DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia. Phillips Petroleum Company, J E Phillips, November 24, 1992: US05166329 (39 worldwide citation)

Method for the site specific genomic modification of yeasts of the genus Pichia and novel DNA sequences useful therefore are provided. Pichia is transformed with a serially arranged linear DNA fragment comprising first and second insertable DNA fragments, which flank a marker gene. The insertable DN ...


10
James M Cregg: Pichia pastoris alcohol oxidase II regulatory region. Phillips Petroleum Company, Hal Brent Woodrow, July 16, 1991: US05032516 (38 worldwide citation)

A novel DNA sequence derived from Pichia pastoris containing a regulatory region from a second alcohol oxidase gene, and novel DNA constructs comprising said regulatory region operably linked to a heterologous DNA structural gene.