1
James Leushner, May Hui, James M Dunn, Jean Michel LaCroix: Method compositions and kit for detection. Visible Genetics, Oppedahl & Larson, April 10, 2001: US06214555 (82 worldwide citation)

Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenaseâ„¢ which incorporates dideoxynucle ...


2
James M Dunn, James Leushner, John Renfrew, Paul Waterhouse, Alexandre M Izmailov, Henryk Zaleski: Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling. Visible Genetics, Oppedahl & Larson, April 27, 1999: US05897842 (64 worldwide citation)

An apparatus and method for thermally cycling a reaction mixture in a reaction vessel to expose the mixture to the varying temperatures necessary to, for example, achieve PCR amplification or the preparation of sequencing fragments using a cycle sequencing operation makes use of flow-through reactio ...


3
John K Stevens, James M Dunn, James Leushner, Ronald J Green: Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types. Visible Genetics, Oppedahl & Larson, November 10, 1998: US05834189 (45 worldwide citation)

The allelic type of a polymorphic genetic locus in a sample is identified by first combining the sample with a sequencing reaction mixture containing a polymerase, nucleotide feedstocks, one type of chain terminating nucleotide and a sequencing primer to form a plurality of oligonucleotide fragments ...


4
James Leushner, May Hui, James M Dunn, Marina T Larson, Jean Michel Lacroix, Robert Shipman: Method for bi-directional sequencing of nucleic acid polymers. Visible Genetics, Oppedahl & Larson, July 4, 2000: US06083699 (36 worldwide citation)

A method is provided for simultaneously determining the positions of a selected nucleotide base in a target region of both strands of a denatured duplex nucleic acid polymer. The nucleic acid polymer is combined with a reactant mixture comprising first and second oligonucleotide primers, said primer ...


5
James Leushner, May Hui, James M Dunn, Marina T Larson: Method for amplification and sequencing of nucleic acid polymers. Visible Genetics, Oppedahl & Larson, August 4, 1998: US05789168 (32 worldwide citation)

Amplification and sequencing of a selected region of a target nucleic acid polymer are be performed in a single vessel. The sample is added to an amplification mixture containing a thermally stable polymerase and nucleoside feedstocks. Chain terminating dideoxynucleosides are added either at the beg ...


6
James Leushner, May Hui, James M Dunn, Marina T Larson: Method for single-tube sequencing of nucleic acid polymers. Visible Genetics, Oppedahl & Larson, November 3, 1998: US05830657 (32 worldwide citation)

Sequencing of a selected region of a target nucleic acid polymer in a genomic DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenase.TM. which incorporates dideoxynucleotides in ...


7
Jean Michel Lacroix, James Leushner, May Hui, James M Dunn, Marina T Larson: Method, compositions and kit for detection and identification of microorganisms. Visible Genetics, Oppedahl & Larson, March 30, 1999: US05888736 (20 worldwide citation)

Evaluation of a sample for the presence and qualitative nature of a microorganism can be performed in a single vessel by combining a natural abundance DNA sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenase.TM. which incorporates dideoxynu ...


8
Robert Shipman, James Leushner, James M Dunn: Method and reagents for testing for mutations in the BRCA1 gene. Visible Genetics, Oppedahl & Larson, June 11, 2002: US06403303 (9 worldwide citation)

Samples are tested for mutations in the BRCA1 gene using a hierarchical approach. First, each sample is amplified in one or more multiplex PCR amplification reactions. Each multiplex PCR reaction produces a mixture of amplified fragments. The sizes and amounts of these fragments are evaluated and co ...


9
James Leushner, Jean Michel Lacroix, May Hui, James M Dunn, Marina T Larson: Method for sequencing of nucleic acid polymers. Visible Genetics, Oppedahl & Larson, July 2, 2002: US06413718 (2 worldwide citation)

Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as Thermo Sequenaseâ„¢ which incorporates dideoxynucleot ...


10
Caroline Elizabeth Garey, James Leushner: Method and kit for amplification, sequencing and typing of classical HLA class I genes. Visible Genetics, Oppedahl & Larson, June 8, 1999: US05910413

Amplification primer and sequencing primer sites have been identified which permit sequence-based typing of each of the classical HLA genes in highly robust and consistent reactions without allelic dropout. To determine the DNA sequence, and thus the type of at least one exon of an HLA-A, HLA-B and ...