1
Erlich Henry Anthony, Saiki Randall Keichi, Horn Glenn Thomas, Mullis Kary Banks: Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids and kits therefor.. Cetus, September 16, 1987: EP0237362-A1 (261 worldwide citation)

A process for detecting the presence or absence of at least one nucleotide variation in sequence in one or more nucleic acids in a sample comprises: (a) treating the sample, together or sequentially with 4 different nucleotide triphosphates (I), an agent (II) for polymerisation of (I), and one oligo ...


2
Mullis Kary Banks, Arnheim Norman, Saiki Randall Keichi, Erlich Henry Anthony, Horn Glenn Thomas, Scharf Stephen Joel: Process for amplifying, detecting, and/or cloning nucleic acid sequences.. Cetus, November 5, 1986: EP0200362-A2 (162 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


3
Erlich Henry Anthony, Horn Glenn, Saiki Randall Keichi, Stoffel Susanne, Mullis Kary Banks, Lawyer Frances Cook, Gelfand David Harrow: Purified thermostable enzyme and process for amplifying, detecting, and/or cloning nucleic acid sequences using said enzyme.. Cetus, March 2, 1988: EP0258017-A2 (151 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


4
Mullis Kary Banks, Arnheim Norman, Saiki Randall Keichi, Erlich Henry Anthony, Horn Glenn Thomas, Scharf Stephen Joel: Kit for use in amplifying and detecting nucleic acid sequences.. Hoffmann La Roche, September 9, 1992: EP0502589-A2 (37 worldwide citation)

The present invention is directed to a kit for the amplification and detection of at least one specific nucleic acid sequence in a sample, which kit comprises in packaged form, a multicontainer unit comprising: (a) primer for each different specific nucleic acid sequence being amplified and detected ...


5
Horn Glenn Thomas, Piatak Michael Jr: Modification of dna sequences.. Cetus, May 17, 1989: EP0316018-A2 (35 worldwide citation)

The invention is embodied by digesting a vector DNA sequence with a restriction enzyme, removing any unwanted nucleotides, treating the cleaved vector with exonuclease III to create 5 min sticky ends, mixing with the thus treated vector an even number of single-stranded nucleotides of alternating se ...


6
Erlich Henry A, Saiki Randall K, Horn Glenn Thomas, Mullis Kary B: Procede de detection de variations specifiques de nucleotides et de polymorphismes genetiques dans les acides nucleiques, Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids. F Hoffmann La Roche, BORDEN LADNER GERVAIS, June 18, 1991: CA1284931 (29 worldwide citation)

Abstract of the Disclosure Single or multiple nucleotide variations in nucleic acidsequence can be detected in nucleic acids by a process whereby thesample suspected of containing the relevant nucleic acid is repeatedlytreated with primers, nucleotide triphosphates, and an agent forpolymerization of ...


7
Greenfield Lawrence I, Nitecki Danute E, Kaplan Donald A, Gelfand David H, Piatak Michael, Horn Glenn, Lawyer Francis Cook: Toxin conjugates.. Cetus, February 12, 1986: EP0170697-A1 (10 worldwide citation)

Toxin conjugates for selective destruction of target cells. Some embodiments employ spacer peptides between the cytotoxic and binding regions of the conjugates to facilitate translocation and intracellular cleavage after binding to the target cell. Components of the conjugate toxins obtained by reco ...


8
Erlich Henry Anthony, Saiki Randall Keichi, Horn Glenn Thomas, Mullis Kary Banks: Primers and probes for nucleic acid detection.. Cetus, December 4, 1991: EP0459532-A2 (7 worldwide citation)

The invention provides an oligonucleotide primer exhibiting complementarity to an end of a nucleic acid sequence corresponding to a second exon sequence in an HLA Class II gene, wherein said primer can be hybridized to said sequence and then extended thereon to produce a single stranded extension pr ...


9
Horn Glenn Thomas, Piatak Michael Jr: Recombinant ricin fragments, vectors and transformed hosts expressing the same, the modification of dna sequences, and isolation of mrna.. Cetus, October 8, 1986: EP0196762-A1 (7 worldwide citation)

Recombinant vectors and methods for constructing ricin B are disclosed. The coding sequence for ricin B was cloned, disposed in suitable expression vectors and produced free of components normally accompanying this peptide. In addition, a novel means of reconstructing missing portions of coding sequ ...


10
Horn Glenn T, Erlich Henry A, Scharf Stephen J, Saiki Randall K, Mullis Kary B: (ou) Cloner des sequences deacides nucleiques, Process for amplifying, detecting and/or cloning nucleic acid sequences. F Hoffmann La Roche, F Hoffmann La Roche, BORDEN LADNER GERVAIS, October 29, 1991: CA1340121 (5 worldwide citation)

The present invention is directed to a process foramplifying and detecting any target nucleic acid sequence contained ina nucleic acid or mixture thereof. The process comprises treatingseparate complementary strands of the nucleic acid with a molar excessof two oligonucleotide primers, extending the ...