1
Shmuel Cabilly, Herbert L Heyneker, William E Holmes, Arthur D Riggs, Ronald B Wetzel: Recombinant immunoglobin preparations. Genentech, March 28, 1989: US04816567 (2766 worldwide citation)

Altered and native immunoglobulins, including constant-variable region chimeras, are prepared in recombinant cell culture. The immunoglobulins contain variable regions which are immunologically capable of binding predetermined antigens. Methods are provided for refolding directly expressed immunoglo ...


2
Shmuel Cabilly, Herbert L Heyneker, William E Holmes, Arthur D Riggs, Ronald B Wetzel: Methods of producing immunoglobulins, vectors and transformed host cells for use therein. Genentech, Burns Doane Swecker & Mathis, December 18, 2001: US06331415 (344 worldwide citation)

The invention relates to processes for producing an immunoglobulin or an immunologically functional immunoglobulin fragment containing at least the variable domains of the immunoglobulin heavy and light chains. The processes can use one or more vectors which produce both the heavy and light chains o ...


3
Herman A Deboer, Rein Strijker, Herbert L Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J A Krimpenfort: Transgenic bovine. Pharming, Townsend & Townsend & Crew, April 21, 1998: US05741957 (168 worldwide citation)

A transgenic bovine is disclosed whose somatic and germ cells contain a transgene, wherein the transgene comprising a mammary gland specific promoter, a mammary gland specific enhancer, a DNA sequence encoding a signal sequence functional in bovine mammary gland secretory cells and a DNA sequence en ...


4
Thomas M Brennan, Herbert L Heyneker: Methods and compositions for determining the sequence of nucleic acids. Richard F Trecartin, April 4, 1995: US05403708 (118 worldwide citation)

Methods and compositions for forming ligation product hybridized to a nucleic acid template. The ligation product is formed by contacting a nucleic acid template with a primer capable of hybridizing to the template to form a primed template. The primed template is then contacted with a pool of rando ...


5
David V Goeddel, Herbert L Heyneker: Method of constructing a replicable cloning vehicle having quasi-synthetic genes. Genentech, Thomas D Kiley, August 3, 1982: US04342832 (88 worldwide citation)

Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products ...


6
Gregory L Gray, Herbert L Heyneker: Secretion of correctly processed human growth hormone in E. coli and Pseudomonas. Genentech, July 5, 1988: US04755465 (82 worldwide citation)

The production of mature hGH In E. coli and Pseudomonas strains transformed by a plasmid which encodes pre hGH (comprising the signal polypeptide and the hormone itself) is described. These prokaryotes process the pre-hGH to cleave the signal sequence and, thereby, produce mature hGH.


7
Gregory L Gray, Herbert L Heyneker: Recombinant procaryotic cell containing correctly processed human growth hormone. Genentech, Max D Hensley, August 22, 1989: US04859600 (58 worldwide citation)

The production of mature hGH in E coli and Pseudomonas strains transformed by a plasmid which encodes pre hGH (comprising the signal polypeptide and the hormone itself) is described. These prokaryotes process the pre-hGH to cleave the signal sequence and, thereby, produce mature hGH.


8
Herbert L Heyneker, Kim D Ha, David K Steinmiller, Victor Simonyi: Apparatus and method for concurrent chemical synthesis. EOS Biotechnology, Townsend & Townsend & Crew, July 24, 2001: US06264891 (52 worldwide citation)

This invention provides an apparatus for preparing chemical libraries. The apparatus includes (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a prog ...


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Chung N Chang, Gregory L Gray, Herbert L Heyneker, Michael W Rey: Secretion of heterologous proteins. Genentech, Robert H Benson, Max D Hensley, October 16, 1990: US04963495 (37 worldwide citation)

Direct linkage of DNA encoding prokaryotic signals such as E. coli enterotoxin with DNA encoding mature eukaryotic proteins followed by transformation and culture of bacterial hosts characterized by a periplasmic space, yields substantial amounts of periplasmic mature protein.