1
Kary B Mullis, Henry A Erlich, Norman Arnheim, Glenn T Horn, Randall K Saiki, Stephen J Scharf: Process for amplifying, detecting, and/or-cloning nucleic acid sequences. Cetus Corporation, Janet E Hasak, Albert P Halluin, July 28, 1987: US04683195 (3538 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


2
Kary B Mullis, Henry A Erlich, David H Gelfand, Glenn Horn, Randall K Saiki: Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halloin, October 23, 1990: US04965188 (1363 worldwide citation)

A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary ...


3
Kary B Mullis, Henry A Erlich, Norman Arnheim, Glenn T Horn, Randall K Saiki, Stephen J Scharf: Process for amplifying, detecting, and/or cloning nucleic acid sequences. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halluin, January 24, 1989: US04800159 (1147 worldwide citation)

The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending ...


4
Randall K Saiki, Henry A Erlich: Method for detection of polymorphic restriction sites and nucleic acid sequences. Cetus Corporation, Janet E Hasak, Albert P Halluin, July 28, 1987: US04683194 (291 worldwide citation)

In a method for detecting the presence or absence of a specific restriction site in a nucleic acid sequence an oligonucleotide probe complementary to one strand of the nucleic acid sequence spanning said restriction site is synthesized. The probe is labeled at the end nearer the restriction site. Th ...


5
Henry A Erlich: HLA typing method and cDNA probes used therein. Cetus Corporation, Albert P Halluin, Janet E Hasak, Thomas E Ciotti, April 15, 1986: US04582788 (179 worldwide citation)

HLA typing based on restriction length polymorphism is carried out by: digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementar ...


6
Henry A Erlich, Russell G Higuchi: Methods for nucleic acid amplification. Hoffman La Roche, George M Gould, Patricia S Rocha, Stacey R Sias, May 24, 1994: US05314809 (142 worldwide citation)

Methods are provided for enhanced specificity and sensitivity of nucleic acid amplification. The methods are simplified nested amplification procedures wherein both inner and outer primer pairs are present in the amplification reaction mixture. According to the methods, the thermocycling profile, as ...


7
Ulf B Gyllensten, Henry A Erlich: Methods for generating single stranded DNA by the polymerase chain reaction. Cetus Corporation, Kevin R Kaster, November 19, 1991: US05066584 (130 worldwide citation)

Single stranded DNA can be generated by the polymerase chain reaction using two oligonucleotide primers, one persent in a limiting concentration. The single stranded DNA is useful in procedures involving utilizing nucleic acid probes and for purposes of nucleic acid sequencing.


8
Henry A Erlich, Glenn Horn, Randall K Saiki, Kary B Mullis: Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids. Hoffmann La Roche, George M Gould, Stacey R Sias, Douglas A Petry, November 21, 1995: US05468613 (87 worldwide citation)

Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and ...


9
Henry A Erlich, Glenn Horn, Randall K Saiki, Kary B Mullis: Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids. Hoffmann La Roche, George W Johnston, Stacey R Sias, Douglas A Petry, February 18, 1997: US05604099 (68 worldwide citation)

Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and ...


10
Henry A Erlich, Glenn Horn, Randall K Saiki, Kary B Mullis, David H Gelfand: Kits for amplifying and detecting nucleic acid sequences. Roche Molecular Systems, Douglas A Petry, March 6, 2001: US06197563 (62 worldwide citation)

The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide prim ...