1
Gale E Smith, Max D Summers: Method for producing a recombinant baculovirus expression vector. The Texas A&M University System, Arnold White & Durkee, May 17, 1988: US04745051 (184 worldwide citation)

A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin p ...


2
Gale E Smith, Max D Summers: Method for producing a recombinant baculovirus expression vector. The Texas A&M University System, Arnold White & Durkee, November 7, 1989: US04879236 (124 worldwide citation)

A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A rec ...


3
Gale E Smith, Franklin Volvovitz, Bethanie E Wilkinson, Andrei I Voznesensky, Craig S Hackett: Vaccine comprising a baculovirus produced influenza hemagglutinin protein fused to a second protein. Protein Sciences Corporation, Thomas J Kowalski, Frommer Lawrence & Haug, January 12, 1999: US05858368 (85 worldwide citation)

A method of preparing a recombinant influenza vaccine using DNA technology is provided. The resulting vaccine is a multivalent, preferably trivalent, influenza vaccine based on a mixture of recombinant hemagglutinin antigens cloned from influenza viruses having epidemic potential. The recombinant he ...


4
Gale E Smith, Franklin Volvovitz, Bethanie E Wilkinson, Andrei I Voznesensky, Craig S Hackett: Method for producing influenza hemagglutinin multivalent vaccines. Protein Sciences Corporation, Thomas J Kowalski, Frommer Lawrence & Haug, June 12, 2001: US06245532 (24 worldwide citation)

A method of preparing a recombinant influenza vaccine using DNA technology is provided. The resulting vaccine is a multivalent, preferably trivalent, influenza vaccine based on a mixture of recombinant hemagglutinin antigens cloned from influenza viruses having epidemic potential. The recombinant he ...


5
Gale E Smith, Harald G Foellmer, John Knell, James DeBartolomeis, Andrei I Voznesensky: Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using. Protein Sciences Corporation, Thomas J Kowalski, Frommer Lawrence & Haug, August 15, 2000: US06103526 (18 worldwide citation)

Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human ...


6
Gale E Smith, John Knell, Andrei I Voznesensky: Apparatus and methods for producing and using high-density cells and products therefrom. Protein Sciences Corporation, Vedder Price P C, Thomas J Kowalski, Deborah L Lu, February 1, 2011: US07879601 (1 worldwide citation)

Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further ...


7
Gale E Smith, John Knell, Andrei I Voznesensky: Apparatus and methods for producing and using high-density cells and products therefrom. Protein Sciences Corporation, Frommer Lawrence & Haug, Thomas J Kowalski, Heather DiPietrantonio, October 6, 2009: US07598075 (1 worldwide citation)

Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further ...


8
Gale E Smith, John Knell, Andrei I Voznesensky: In vivo active erythropoietin produced in insect cells. Protein Sciences Corporation, Thomas J Kowalski, Deborah L Lu, Vedder Price PC, September 27, 2011: US08026096

Disclosed and claimed is a human erythropoietin (EPO) expressed and produced in Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579) transfected with a baculovirus construct containing the EPO gene. The EPO has an in vivo activity of 200,000 U/mg to 500,000 U/mg.


9
Franklin Volvovitz, Gale E Smith, Mark A Cochran: Polypeptides derived from envelope gene of human immunodeficiency virus in recombinant baculovirus infected insect cells. Microgenesys, West Haven, Connecticut 06516, Usa, YANG JIUCHANG, December 21, 1988: CN87107837

The baculovirus-insect cell vector system is used as a high efficiency eukaryotic cloning and expression method for the production of recombinant proteins. A major application of this system is also in the development of rDNA vaccines for a variety of important human and animal diseases. This system ...


10
Gale E Smith, Jane A Thanassi, Mark A Cochran: Recombinant japanese encephalitis molecules and methods of use therefor. Micro Gene Sys, YANG JIUCHANG, June 26, 1991: CN90107937

This invention provides recombinant Japanese encephalitis DNA molecules, vectors and the recombinant proteins which are produced by them. This invention also provides methods for detecting immunizing and treating Japanese encephalitis infection in a subject which comprises using the recombinant Japa ...



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