1
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Janet E Hasak, Kevin R Kaster, Albert P Halluin, December 26, 1989: US04889818 (432 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


2
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Lisabeth F Murphy, Kevin R Kaster, January 7, 1992: US05079352 (185 worldwide citation)

Recombinant DNA vectors that encode a thermostable DNA polymerase are useful in the recombinant production of thermostable DNA polymerase. The recombinant thermostable polymerase is preferred for use in the production of DNA in a polymerase chain reaction. Especially useful vectors encode the .about ...


3
David H Gelfand, Frances C Lawyer: DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, December 20, 1994: US05374553 (97 worldwide citation)

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a ...


4
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase. Hoffmann La Roche, George W Patricia S Rocha Tramaloni Johnston, Stacey R Sias, April 8, 1997: US05618711 (70 worldwide citation)

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an .about.94 kDa protein especially useful in the DNA amplification procedu ...


5
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Purified thermostable nucleic acid polymerase enzyme from Thermotoga maritima. Hoffmann La Roche, George W Johnston, Patricia S Rocha Tramaloni, Stacey R Sias, April 29, 1997: US05624833 (53 worldwide citation)

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with prim ...


6
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Mutated thermostable nucleic acid polymerase enzyme from thermotoga maritima. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, May 30, 1995: US05420029 (49 worldwide citation)

A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight as determined by gel electrophoresis of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with prim ...


7
David H Gelfand, Frances C Lawyer: Portable temperature-sensitive control cassette. Cetus Corporation, Kate H Murashige, Albert P Halluin, December 8, 1987: US04711845 (47 worldwide citation)

Two types of convenient portable control cassettes for the expression of protein encoding sequences in procaryotes are disclosed. Both cassettes comprise the P.sub.L promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome bindi ...


8
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Recombinant expression vectors and purification methods for thermus thermophilus DNA polymerase. Roche Molecular Systems, George W Johnston, Dennis P Tramaloni, Douglas A Petry, August 4, 1998: US05789224 (37 worldwide citation)

Recombinant DNA sequences encoding the DNA polymerase activity of Thermus thermophilus can be used to construct recombinant vectors and transformed host cells for production of the activity. T. thermophilus DNA polymerase is an .about.94 kDa protein especially useful in the DNA amplification procedu ...


9
David H Gelfand, Lawrence I Greenfield, Frances C Lawyer: Recombinant diphtheria toxin fragments. Cetus Corporation, Albert P Halluin, Elliott L Fineman, Thomas E Ciotti, May 16, 1989: US04830962 (25 worldwide citation)

Recombinant vectors which are effective in expressing DNA sequences encoding specific fragments of diphtheria toxins at high levels in recombinant host cells are disclosed. Both a fragment consisting of the enzymatically active A chain of diphtheria toxin and a fragment consisting of both the A port ...


10
David H Gelfand, Frances C Lawyer, Susanne Stoffel: Universal dominant selectable marker cassette. Cetus Corporation, Albert P Halluin, Kate H Murashige, November 15, 1988: US04784949 (24 worldwide citation)

A novel universal dominant selectable marker cassette is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which has been modified and truncated so as to render its use in recombinant vectors more convenient. The modified, truncated sequence (mtAPH- ...