1
Eric B Kmiec: Compounds and methods for site directed mutations in eukaryotic cells. Thomas Jefferson University, Pennie & Edmonds, October 15, 1996: US05565350 (346 worldwide citation)

The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single ...


2
Eric B Kmiec, Howard B Gamper, Michael C Rice: Targeted chromosomal genomic alterations with modified single stranded oligonucleotides. University of Delaware, Heller Ehrman White & McAuliffe, Daniel M Becker, August 30, 2005: US06936467 (81 worldwide citation)

Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides. The oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base a ...


3
Eric B Kmiec, Howard B Gamper, Michael C Rice: Targeted chromosomal genomic alterations with modified single stranded oligonucleotides. University of Delaware, McCarter & English, June 5, 2007: US07226785 (74 worldwide citation)

Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base analogs.


4
Eric B Kmiec: Chimeric mutational vectors having non-natural nucleotides. Thomas Jefferson University, Daniel Hansburg, March 24, 1998: US05731181 (60 worldwide citation)

The application concerns the design and use of small, duplex oligonucleotides and oligomers of nucleotide analogs, termed Chimeric Mutational Vectors (CMV) to effect mutation in a target gene of a eukaryotic cell by homologous recombination between the CMV and the target. The CMV comprises ribonucle ...


5
Eric B Kmiec: Compounds and methods for site directed mutations in eukaryotic cells. Thomas Jefferson University, Joan E Switzer, Daniel Hansburg, Thomas E Friebel, May 26, 1998: US05756325 (58 worldwide citation)

The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single ...


6
Eric B Kmiec, Allyson Cole Strauss, Kyonggeun Yoon: Methods and compounds for curing diseases caused by mutations. Thomas Jefferson University, Daniel Hansburg, Thomas E Friebel, June 2, 1998: US05760012 (57 worldwide citation)

The invention concerns the use of duplex oligonucleotides having both 2'-deoxyribonucleotides and ribonucleotides, wherein there is base pairing between the two types of nucleotides. The sequence of the oligonucleotide is selected so that the 3' and 5' most regions of the oligonucleotide are homolog ...


7
Eric B Kmiec, Howard B Gamper, Allyson D Cole Strauss: Eukaryotic use of non-chimeric mutational vectors. Kimeragen, Pennie & Edmonds, January 4, 2000: US06010907 (45 worldwide citation)

The invention is based on the reaction of Duplex Mutational Vector in a cell-free system containing a cytoplasmic cell extract and a test plasmid. The reaction specifically converts a mutant kan.sup.r gene to recover the resistant phenotype in transformed MutS, RecA deficient bacteria. Using this sy ...


8
Eric B Kmiec: Chimeric mutational vectors having non-natural nucleotides. Thomas Jefferson University, Daniel Hansburg, August 18, 1998: US05795972 (40 worldwide citation)

The application concerns the design and use of small, duplex oligonucleotides and oligomers of nucleotide analogs, termed Chimeric Mutational Vectors (CMV) to effect mutation in a target gene of a eukaryotic cell by homologous recombination between the CMV and the target. The CMV comprises ribonucle ...


9
William K Holloman, Eric B Kmiec: Methods to promote homologous recombination in eukaryotic cells and organisms. Cornell Research Foundation, Thomas Jefferson University, Daniel Hansburg, August 31, 1999: US05945339 (39 worldwide citation)

The invention concerns genes encoding recombinases that can be used to promote homologous recombination in eukaryotic cells. The application teaches methods by which a recombinase of one species can be used to isolate a homologous recombinase of a different species and methods to identify the isolat ...


10
Eric B Kmiec: Compounds and methods for site directed mutations in eukaryotic cells. Thomas Jefferson University, Daniel Hansburg, February 16, 1999: US05871984 (35 worldwide citation)

The present invention concerns a polynucleotide having both ribonucleotides and deoxyribonucleotides in a first strand and solely deoxyribonucleotides in a second strand; wherein the strands are Watson-Crick paired and are linked by an oligonucleotide so that the polynucleotide has at most a single ...