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G Richard Cathcart, Thomas Brennan Marquez, John A Bridgham, George S Golda, Harry A Guiremand, Marianne Hane, Louis B Hoff, Eric Lachenmeier, Melvyn N Kronick, Douglas H Keith, Paul E Mayrand, Michael L Metzker, William J Mordan, Lincoln J McBride, John Shigeura, Chen Hanson Ting, Norman M Whiteley: Automated molecular biology laboratory. Perkin Elmer Applied Biosystems Division, Donald R Boys, Joseph H Smith, August 22, 1995: US05443791 (318 worldwide citation)

A liquid-handling instrument has a worksurface with registration for modular stations to support containers of liquid, pipette apparatus with a pipette tip coupled to a sensing circuit, a robotic translation system for moving the pipette tip, and a control system with an iconic user interface for pr ...


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Douglas H Keith, Mel N Kronick, Lincoln J McBride, Norman M Whiteley: Labeling by simultaneous ligation and restriction. Applied Biosystems, Cooley Godward Castro Huddleson & Tatum, March 3, 1992: US05093245 (51 worldwide citation)

Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of th ...


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Douglas H Keith: Restriction/ligation labeling for primer initiated multiple copying of DNA ssequences. Applied Biosystems, Richard Neeley, November 22, 1994: US05366877 (27 worldwide citation)

Sample DNA is analyzed by joining dsDNA sample fragments to labeling moieties having a primer binding sequence, to provide labeled dsDNA. After denaturation of the labeled dsDNA, strands binding to a probe are separated, conveniently using particles and a specific binding pair, followed by amplifica ...


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Douglas H Keith: Method of detecting specific substances by selective growth of living cells. City of Hope, Edward S Irons, February 4, 1992: US05085982 (11 worldwide citation)

The invented method utilizes a signal amplification system comprising living cells which are specifically provided with the ability to survive, reproduce and be detected in the event that a target molecule is present. The method comprises first the step of binding a target molecule to a substratum. ...


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Mel N Kronick, Douglas H Keith, Lincoln J McBride, Norman M Whiteley, Michael W Hunkapiller: Nucleic acid detection with separation. Applied Biosystems, Cooley Godward, April 25, 2000: US06054266 (6 worldwide citation)

Nucleic acid sequences are detected by a multi-step process, involving labeling sample nucleic acid sequences, duplexing the labeled sample with a probe having a coupling element, immobilizing all of the duplexed probe and target sequence and unduplexed probe, separating specifically immobilized nuc ...