1
Kary B Mullis, Henry A Erlich, David H Gelfand, Glenn Horn, Randall K Saiki: Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme. Cetus Corporation, Kevin R Kaster, Janet E Hasak, Albert P Halloin, October 23, 1990: US04965188 (1350 worldwide citation)

A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary ...


2
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Homogeneous assay system using the nuclease activity of a nucleic acid polymerase. Hoffman La Roche, George M Gould, Dennis P Tramaloni, Stacev R Sias, May 11, 1993: US05210015 (628 worldwide citation)

The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide frag ...


3
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Janet E Hasak, Kevin R Kaster, Albert P Halluin, December 26, 1989: US04889818 (426 worldwide citation)

A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling ch ...


4
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Nucleic acid detection by the 5-3exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides. Hoffmann La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, January 30, 1996: US05487972 (264 worldwide citation)

A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is e ...


5
David H Gelfand: Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription. Hoffman LaRoche, George M Gould, Dennis P Tramaloni, Stacey RG351 5 Sias, June 21, 1994: US05322770 (196 worldwide citation)

Methods are provided for the replication and amplification of RNA sequences by thermostable DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 92 kDa Taq, 64 kDa Taq, and Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure usin ...


6
David H Gelfand, Pamela M Holland, Randall K Saiki, Robert M Watson: Reaction mixtures for detection of target nucleic acids. Roche Molecular Systems, George W Johnston, Patricia S Rocha Tramaloni, Stacey R Sias, September 8, 1998: US05804375 (194 worldwide citation)

A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorpo ...


7
David H Gelfand, Susanne Stoffel, Frances C Lawyer, Randall K Saiki: Purified thermostable enzyme. Cetus Corporation, Lisabeth F Murphy, Kevin R Kaster, January 7, 1992: US05079352 (183 worldwide citation)

Recombinant DNA vectors that encode a thermostable DNA polymerase are useful in the recombinant production of thermostable DNA polymerase. The recombinant thermostable polymerase is preferred for use in the production of DNA in a polymerase chain reaction. Especially useful vectors encode the .about ...


8
David H Gelfand, Shirley Y Kwok, John J Sninsky: Reduction of non-specific amplification glycosylase using DUTP and DNA uracil. Hoffmann La Roche, George M Gould, Stacey R Sias, Douglas A Petry, May 23, 1995: US05418149 (143 worldwide citation)

Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bags in ...


9
David H Gelfand, Thomas W Myers: Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription. Hoffman La Roche, George M Gould, Dennis P Tramaloni, Stacey R Sias, May 10, 1994: US05310652 (131 worldwide citation)

Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for ...


10
Michael A Innis, Kenneth B Myambo, David H Gelfand, Mary Ann D Brow: Methods for DNA sequencing with thermus aquaticus DNA polymerase. Cetus Corporation, Stacey R Sias, Kevin R Kaster, December 24, 1991: US05075216 (129 worldwide citation)

Dideoxynucleotide DNA sequencing methods can be dramatically improved by utilizing the DNA polymerase from Thermus aquaticus to catalyze the primer extension reactions.