1
Danute E Nitecki: Preparation of an activated polymer ester for protein conjugation. Cetus Oncology Corporation, Grant D Green, Kenneth M Goldman, Philip L McGarrigle Jr, January 25, 1994: US05281698 (226 worldwide citation)

The present invention is a process for preparing an activated ester of polyethylene glycol or a polyoxyethylated polyol. After the activated ester is prepared, it can be reacted with a protein to form a polymer/protein conjugate. Conjugation with a polymer can reduce the protein's immunogenicity, in ...


2
Danute E Nitecki, Nandini Katre, Robert J Goodson, Lois Aldwin: Preparation of a polymer/interleukin-2 conjugate. Cetus Corporation, Philip L McGarrigle, Albert P Halluin, February 20, 1990: US04902502 (130 worldwide citation)

The present invention is a process for preparing a pharmaceutical composition comprising a biologically active conjugated protein. It comprises a polyethylene glycol or a polyoxyethylated polyol conjugated to IL-2. This protein is conjugated to reduce its immunogenicity, and increase it solubility, ...


3
Paula J Shadle, Kirston E Koths, Margaret Moreland, Nandini Katre, Walter J Laird, Lois Aldwin, Danute E Nitecki, John D Young: Conjugation of polymer to colony stimulating factor-1. Cetus Corporation, Philip L McGarrigle, Jane R McLaughlin, Albert P Halluin, October 6, 1992: US05153265 (92 worldwide citation)

A biologically active CSF-1 protein is selectively conjugated via certain amino acid residues or carbohydrate moieties to a water-soluble polymer selected from polyethylene glycol or polypropylene glycol homopolymers, polyoxyethylated polyols, or polyvinyl alcohol. The resulting conjugated CSF-1 is ...


4
Danute E Nitecki, Nandini Katre, Robert J Goodson, Lois Aldwin: Preparation of a polymer/interleukin-2 conjugate. Cetus Corporation, Philip L McGarrigle, February 18, 1992: US05089261 (89 worldwide citation)

The present invention is a process for preparing a pharmaceutical composition comprising a biologically active conjugated protein. It comprises a polyethylene glycol or a polyoxyethylated polyol conjugated to IL-2. This protein is conjugated to reduce its immunogenicity, and increase its solubility, ...


5
Lawrence I Greenfield, Donald A Kaplan, Danute E Nitecki: Toxin conjugates. Cetus Corporation, Kate H Murashige, Gregory J Giotta, Albert P Halluin, January 16, 1990: US04894443 (79 worldwide citation)

A novel class of polypeptides of the general formula (F-(Pro).sub.n).sub.m F, wherein F represents a flexible amino acid sequence wherein each amino acid is individually selected from the group consisting of serine, glycine, and threonine, and n is an integer from 4-8 inclusive and m is an integer f ...


6
Margaret Moreland, Danute E Nitecki: Solubilization of proteins for pharmaceutical compositions using heparin fragments. Cetus Corporation, Janet E Hasak, Albert P Halluin, May 17, 1988: US04745180 (54 worldwide citation)

A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is poorly or not at all water ...


7
Lois Aldwin, Danute E Nitecki: Solubilization of proteins for pharmaceutical compositions using polyproline conjugation. Cetus Corporation, Philip L McGarrigle, Janet E Hasak, Albert P Halluin, January 16, 1990: US04894226 (38 worldwide citation)

A pharmaceutical composition is prepared wherein a biologically active conjugated protein is dissolved in an aqueous carrier medium in the absence of a solubilizing agent. The unconjugated protein, which is not readily water-soluble at pH 6-8 without such solubilizing agent, is covalently conjugated ...


8
Danute E Nitecki, Pradip K Bhatnagar: Method of conjugating oligopeptides. The Regents of the University of California, Bertram I Rowland, December 11, 1984: US04487715 (28 worldwide citation)

Novel methods are provided for preparing peptide compounds involving functionalizing an available amino group with a carboxy group, esterifying the carboxy group with an hydroxylic compound which forms an ester which is stable under the conditions of cleavage of the oligopeptide from said resin, but ...


9
L L Houston, Lois Aldwin, Danute E Nitecki: Thioether linked immunotoxin conjugates. Cetus Corporation, Gregory J Giotta, Albert P Halluin, June 18, 1991: US05024834 (23 worldwide citation)

Heterobifunctional crosslinkers up to about 34 .ANG. in length consisting of a sulfhydryl reactive group linked to a spacer group, which in turn is linked to an activated carboxylate group, that are useful for making efficacious anticancer immunotoxin conjugates as shown preferably by reacting an an ...


10
Francis P McCormick, Gail L Wong, Robin Clark, Norman Arnheim, Danute E Nitecki, James R Feramisco: Peptide antibodies and their use in detecting oncogene products. Cetus Corporation, Cold Spring Harbor Laboratories, Albert P Halluin, Lisabeth F Murphy, Vanet E Hasak, August 9, 1988: US04762706 (22 worldwide citation)

Antibodies exhibit specificity toward single amino acid differences between proteins. These antibodies may be produced by synthesizing a peptide of the appropriate amino acid sequence contained in the protein, immunizing a host with the peptide, and extracting sera from the host to obtain the antibo ...