1
Steven M Menchen, Linda G Lee, Charles R Connell, N Davis Hershey, Vergine Chakerian, Sam Woo, Steven Fung: 4,7-dichlorofluorescein dyes as molecular probes. Applied Biosystems, Stephen C Macevicz, Joseph H Smith, February 23, 1993: US05188934 (231 worldwide citation)

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are describe ...


2
Timothy M Woudenberg, Kevin S Bodner, Charles R Connell, Alan M Ganz, Lincoln J McBride, Paul G Saviano, John Shigeura, David H Tracy, Eugene F Young, Linda G Lee: System for real time detection of nucleic acid amplification products. The Perkin Elmer Corporation Applied Biosystems Division, Wilson Sonsini Goodrich & Rosati, July 27, 1999: US05928907 (205 worldwide citation)

A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing (i) a first fluorescent indicat ...


3
Michael W Hunkapiller, Charles R Connell, William J Mordan, John D Lytle, John A Bridgham: Real time scanning electrophoresis apparatus for DNA sequencing. Applied Biosystems, Joseph H Smith, March 7, 1989: US04811218 (172 worldwide citation)

A real-time, automated, nucleic acid sequencing apparatus that offers high speed, definitive sequencing on many samples at the same time. The apparatus permits more than one clone to be sequenced at a time, thus vastly decreasing the time required to sequence longer fragments and reducing sequencing ...


4
Steven Fung, Sam L Woo, Richard P Haugland, Steven M Menchen, Charles R Connell: Method of detecting electrophoretically separated oligonucleotides. Applied Biosystems, Steven C Macevicz, Joseph H Smith, August 8, 1989: US04855225 (162 worldwide citation)

A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from ...


5
B John Bergot, Vergine Chakerian, Charles R Connell, J Scott Eadie, Steven Fung, N Davis Hershey, Linda G Lee, Steven M Menchen, Sam L Woo: Spectrally resolvable rhodamine dyes for nucleic acid sequence determination. Applied Biosystems, Stephen C Macevicz, Peter Dehlinger, Vince Powers, November 22, 1994: US05366860 (157 worldwide citation)

A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucle ...


6
Lloyd M Smith, Leroy E Hood, Michael W Hunkapiller, Tim J Hunkapiller, Charles R Connell: Automated DNA sequencing technique. California Institute of Technology, Morrison & Foerster, October 13, 1998: US05821058 (116 worldwide citation)

A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electro ...


7
Timothy M Woudenberg, Kevin S Bodner, Charles R Connell, John Shigeura, David H Tracy, Eugene F Young: Apparatus and method for detecting nucleic acid amplification products. Perkin Elmer Corporation Applied Biosystems Division, David J Wilson Sonsini Goodrich & Rosati Weitz, January 18, 2000: US06015674 (107 worldwide citation)

An apparatus is provided which includes a sample holder for holding a reaction chamber which includes an optical interface, a fiber optic cable for delivering an excitation beam to a sample housed within the reaction chamber and for receiving light emitted by the sample, and a lens co-axially dispos ...


8
Steven M Menchen, Linda G Lee, Charles R Connell, N Davis Hershey, Vergine Chakerian, Sam L Woo, Steven Fung: 4,7-dichlorofluorescein dyes as molecular probes. The Perkin Elmer Corporation, Stephen C Macevicz, Vincent M Powers, August 5, 1997: US05654442 (23 worldwide citation)

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5',7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA ...


9
Timothy M Woudenberg, Dar Bahatt, Muhammad A Sharaf, Timothy Z Liu, Serguei Ermakov, Charles R Connell, Jens J Hyldig Nielsen: Multiplex detection compositions, methods, and kits. Applera Corporation, John W Burns, April 3, 2007: US07198900 (12 worldwide citation)

The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determ ...


10
Steven M Menchen, Linda G Lee, Charles R Connell, N Davis Hershey, Vergine Chakerian, Sam L Woo, Steven Fung: 4,7-dichlorofluorescein dyes as molecular probes. The Perkin Elmer Corporation, Vincent M Powers, Stephan C Macevicz, August 1, 2000: US06096723 (12 worldwide citation)

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2',4',5', 7'-tetrachloro-4,7-dichloro-5- (and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DN ...