1
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: Monitoring amplification of DNA during PCR. University of Utah Research Foundation, Barnes & Thornburg, January 16, 2001: US06174670 (363 worldwide citation)

Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Method ...


2
Carl T Wittwer, David R Hillyard, Kirk M Ririe: Rapid thermal cycling device. University of Utah Research Foundation, Thorpe North & Western, October 3, 1995: US05455175 (147 worldwide citation)

A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and the ...


3
Carl T Wittwer, Philip S Bernard: Multiplex genotyping using fluorescent hybridization probes. University of Utah Research Foundation, Barnes & Thornburg, October 31, 2000: US06140054 (132 worldwide citation)

The present invention is directed to a mutation detection kit and method of analyzing multiple loci of one or more nucleic acid sequences for the presence of mutations or polymorphisms. More particularly, the present invention relates to the use of the polymerase chain reaction (PCR) and fluorescent ...


4
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: Monitoring hybridization during PCR using SYBR™ Green I. University of Utah Research Foundation, Barnes & Thornburg, May 27, 2003: US06569627 (106 worldwide citation)

The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a double-stranded nucleic acid binding dye capable of p ...


5
Carl T Wittwer, Mark G Herrmann: Homogeneous multiplex hybridization analysis by color and Tm. The University of Utah, Richard F Trecartin, Dorsey & Whitney, October 29, 2002: US06472156 (105 worldwide citation)

The invention provides methods and devices for analyzing sequence variations in nucleic acid samples comprising multiple loci, each having two, three or more possible allelic sequences. The method involves combining at least a first and second pair of oligonucleotide probes with the nucleic acid sam ...


6
Carl T Wittwer, Martin Gutekunst, Sabine Lohmann: Method for quantification of an analyte. Roche Diagnostics, University of Utah Research Foundation, Barnes & Thornburg, October 16, 2001: US06303305 (85 worldwide citation)

The invention provides a new method for the quantification of a nucleic acid, wherein in a first step, the nucleic acid is amplified by an amplifying agent. Subsequently, first, second or n th order derivatives is calculated. The obtained value can then be used to calculate the initial concentration ...


7
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen: PCR method for nucleic acid quantification utilizing second or third order rate constants. University of Utah Research Foundation, Barnes & Thornburg, May 15, 2001: US06232079 (65 worldwide citation)

The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of am ...


8
Carl T Wittwer, David R Hillyard: Method for rapid thermal cycling of biological samples. The University of Utah, Richard F Trecartin, Dorsey & Whitney, September 7, 2004: US06787338 (59 worldwide citation)

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are place ...


9
Carl T Wittwer, Kirk M Ririe, Randy P Rasmussen, David R Hillyard: System and method for fluorescence monitoring. University of Utah Research Foundation, Barnes & Thornburg, July 25, 2006: US07081226 (51 worldwide citation)

A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are plac ...


10
Harold P Swerdlow, Carl T Wittwer: On-line DNA analysis system with rapid thermal cycling. University of Utah Research Foundation, Thorpe North & Western L, August 10, 1999: US05935522 (50 worldwide citation)

An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a ...